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Methylene Tetrahydrofolate Dehydrogenase 2 (MTHFD2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627753

MTHFD2 is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and… More details

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Product Overview

Online Datasheet in PDF
Product Name
Methylene Tetrahydrofolate Dehydrogenase 2 (MTHFD2), Polyclonal Antibody
Full Product Name
Methylene Tetrahydrofolate Dehydrogenase 2, CT (MTHFD2, Bifunctional Methylenetetrahydrofolate Dehydrogenase/cyclohydrolase Mitochondrial, NADP+ Dependent Methylenetetrahydrofolate Dehydrogenase 2 Methenyltetrahydrofolate Cyclohydrolase, NMDMC)
Product Gene Name
anti-MTHFD2 antibody
Product Synonym Names
Anti -Methylene Tetrahydrofolate Dehydrogenase 2, CT (MTHFD2, Bifunctional Methylenetetrahydrofolate Dehydrogenase/cyclohydrolase Mitochondrial, NADP+ Dependent Methylenetetrahydrofolate Dehydrogenase 2 Methenyltetrahydrofolate Cyclohydrolase, NMDMC)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human MTHFD2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
37,895 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (74425690..74442425). Location: 2p13.1
OMIM
604887
3D Structure
ModBase 3D Structure for P13995
Immunogen
Synthetic peptide selected from the C-terminal region of human MTHFD2 (KLH).

Product Categories/Family for anti-MTHFD2 antibody

Antibodies; Abs to Enzymes; Dehydrogenase
Applications Tested/Suitable for anti-MTHFD2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-MTHFD2 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-MTHFD2 antibody

Western blot analysis of MTHFD2 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). MTHFD2 (arrow) was detected using the purified Pab.
Rabbit Anti-MTHFD2 Polyclonal Antibody – Cat# MBS627753

Immunohistochemistry (IHC) of anti-MTHFD2 antibody

Formalin-fixed and paraffin-embedded human Colon carcinoma reacted with MTHFD2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-MTHFD2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627753

Flow Cytometry (FC/FACS) of anti-MTHFD2 antibody

MTHFD2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MTHFD2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS627753

Other Notes

Small volumes of anti-MTHFD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MTHFD2 purchase

MBS627753 is an antibody targeting MTHFD2. Also known as Methylene Tetrahydrofolate Dehydrogenase 2, CT (MTHFD2, Bifunctional Methylenetetrahydrofolate Dehydrogenase/cyclohydrolase Mitochondrial, NADP+ Dependent Methylenetetrahydrofolate Dehydrogenase 2 Methenyltetrahydrofolate Cyclohydrolase, NMDMC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MTHFD2. It may not necessarily be applicable to this product.

NCBI GI #
62897643
NCBI GeneID
10797
UniProt Primary Accession #
P13995
UniProt Secondary Accession #
Q53G90; Q53GV5; Q53S36
UniProt Related Accession #
P13995; Q7Z650
NCBI Official Full Name
methylene tetrahydrofolate dehydrogenase 2 variant
NCBI Official Synonym Full Names
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
NCBI Official Symbol
MTHFD2
NCBI Official Synonym Symbols
NMDMC
UniProt Protein Name
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Protein Family
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase
UniProt Gene Name
MTHFD2
UniProt Synonym Gene Names
NMDMC
UniProt Entry Name
MTDC_HUMAN
NCBI Protein Information
bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; OTTHUMP00000160246; OTTHUMP00000203020; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase

NCBI Summary for MTHFD2

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]
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UniProt Comments for MTHFD2

MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Protein type: EC 3.5.4.9; Mitochondrial; Cofactor and Vitamin Metabolism - one carbon pool by folate; Hydrolase; EC 1.5.1.15; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular space; mitochondrion; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; magnesium ion binding; methylenetetrahydrofolate dehydrogenase (NAD+) activity; phosphate binding; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity

Biological Process: tetrahydrofolate metabolic process; folic acid and derivative biosynthetic process; one-carbon compound metabolic process
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Research Articles on MTHFD2

1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MTHFD2 antibody

 Products by Pathway  Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Nucleotide Metabolism Pathway antibodies  Nucleotide Metabolism Pathway Diagram
 One Carbon Metabolism Pathway antibodies  One Carbon Metabolism Pathway Diagram
 One Carbon Pool By Folate Pathway antibodies  One Carbon Pool By Folate Pathway Diagram
 One Carbon Pool By Folate Pathway antibodies  One Carbon Pool By Folate Pathway Diagram

Diseases associated with anti-MTHFD2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with MTHFD2 and Neoplasms
 Congenital Abnormalities Antibodies  >2 publications with MTHFD2 and Congenital Abnormalities
 Neural Tube Defects Antibodies  >2 publications with MTHFD2 and Neural Tube Defects
 Nervous System Malformations Antibodies  >2 publications with MTHFD2 and Nervous System Malformations
 Neoplasm Metastasis Antibodies  >1 publications with MTHFD2 and Neoplasm Metastasis
 Heart Defects, Congenital Antibodies  >1 publications with MTHFD2 and Heart Defects, Congenital
 Liver Diseases Antibodies  >1 publications with MTHFD2 and Liver Diseases
 Urinary Bladder Neoplasms Antibodies  >1 publications with MTHFD2 and Urinary Bladder Neoplasms
 Neurodegenerative Diseases Antibodies  >1 publications with MTHFD2 and Neurodegenerative Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with MTHFD2 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-MTHFD2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >2 publications with MTHFD2 and Liver
 Embryonic Tissue Antibodies  >2 publications with MTHFD2 and Embryonic Tissue
 Nerve Antibodies  >1 publications with MTHFD2 and Nerve
 Muscle Antibodies  >1 publications with MTHFD2 and Muscle
 Heart Antibodies  >1 publications with MTHFD2 and Heart
 Bladder Antibodies  >1 publications with MTHFD2 and Bladder
 Brain Antibodies  >1 publications with MTHFD2 and Brain
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