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Histone H3 (H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627810

MBS627810 is an antibody targeting H3. Also known as Histone H3, phosphorylated (Thr80), trimethyl (Lys79). Reactive in Human. Host: Rabbit. More details

$840 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H3, Polyclonal Antibody
Full Product Name
Histone H3, phosphorylated (Thr80), trimethyl (Lys79)
Product Gene Name
Product Synonym Names
Anti -Histone H3, phosphorylated (Thr80), trimethyl (Lys79)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes histone H3 trimethylated at lysine 79 and phosphorylated at threonine 80, Mr 17kD. Species Crossreactivity: Human. Broad species crossreactivity is expected.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
KLH-conjugated, synthetic peptide containing (…[me3K][pT]…), in which me3K corresponds to trimethyl-lysine 79 and pT corresponds to phosphothreonine 80 of human histone H3 and histone H4.

Product Categories/Family for anti-H3 antibody

Applications Tested/Suitable for anti-H3 antibody
Western Blot (WB)
Application Notes for anti-H3 antibody
Suitable for use in Western Blot and Peptide Inhibition.
Dilution: Western Blot: 1:5,000-1:10,000 detects histone H3 in acid-extracted proteins from HeLa cells arrested with colcemid to a greater extent than in untreated HeLa cells. Does not detect unmodified recombinant Histone H3. Recombinant Histone H3 and acid-extracted proteins from untreated and colcemid-arrested HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-trimethyl (Lys79)-phospho (Thr80) Histone H3 (1:1,000 dilution). Proteins were visualized using a goat antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Western Blot (WB) of anti-H3 antibody

WB: Recombinant Histone H3 (14-411, Lane 1) and acid-extracted proteins from untreated (Lane 2) and colcemid-arrested (Lane 3) HeLa cells (17-306) were probed with MBS627810 Lys79)-phospho (Thr80) Histone H3 (1:1,000 dilution).
Rabbit Anti-H3 Polyclonal Antibody – Cat# MBS627810

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
Protein Family
NCBI Protein Information
histone H3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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