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BEST2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6278262

BEST2 is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BEST2, Polyclonal Antibody
Full Product Name
BEST2, CT (BEST2, VMD2L1, Bestrophin-2, Vitelliform macular dystrophy 2-like protein 1) (PE)
Product Gene Name
Product Synonym Names
BEST2; VMD2L1; Bestrophin-2; Vitelliform macular dystrophy 2-like protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
57,139 Da
OMIM
607335
3D Structure
ModBase 3D Structure for Q8NFU1
Immunogen
BEST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381~411 amino acids from the C-terminal region of human BEST2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-BEST2 antibody

Applications Tested/Suitable for anti-BEST2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-BEST2 antibody
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-BEST2 antibody

Western blot analysis of BEST2 Antibody (C-term) in MDA-MB435,MDA-MB231 cell line lysates (35ug/lane).BEST2 (arrow) was detected using the purified Pab.
Rabbit Anti-BEST2 Polyclonal Antibody – Cat# MBS6278262

Immunohistochemistry (IHC) of anti-BEST2 antibody

Formalin-fixed and paraffin-embedded human breast carcinoma with BEST2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-BEST2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6278262

Product References and Citations for anti-BEST2 antibody

Zhang, Y., et.al, Mol. Vis. 16, 200-206 (2010). Marsey, L.L. et.al, J. Physiol. (Lond.) 587 (PT 10), 2211-2224 (2009).

Other Notes

Small volumes of anti-BEST2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BEST2 purchase

MBS6278262 is an antibody targeting BEST2. Also known as BEST2, CT (BEST2, VMD2L1, Bestrophin-2, Vitelliform macular dystrophy 2-like protein 1) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BEST2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bestrophin-2
NCBI Official Synonym Full Names
bestrophin 2
NCBI Official Symbol
BEST2
NCBI Official Synonym Symbols
VMD2L1
UniProt Protein Name
Bestrophin-2
UniProt Synonym Protein Names
Vitelliform macular dystrophy 2-like protein 1
Protein Family
UniProt Gene Name
BEST2
UniProt Synonym Gene Names
VMD2L1
UniProt Entry Name
BEST2_HUMAN
NCBI Protein Information
bestrophin-2; VMD2-like gene 1; vitelliform macular dystrophy 2-like 1; vitelliform macular dystrophy 2-like protein 1

NCBI Summary for BEST2

This gene is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif. Mutation in one of the family members (bestrophin 1) is associated with vitelliform macular dystrophy. The bestrophin 2 gene is mainly expressed in the retinal pigment epithelium and colon. [provided by RefSeq, Jul 2008]
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UniProt Comments for BEST2

BEST2: Forms calcium-sensitive chloride channels. Permeable to bicarbonate. Belongs to the bestrophin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: plasma membrane; cilium

Molecular Function: chloride channel activity

Biological Process: membrane depolarization; sensory perception of smell
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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