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RCL1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627927

RCL1, which does not have cyclase activity, plays a role in 40S- ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RCL1, Polyclonal Antibody
Full Product Name
RCL1, NT (RNA 3'-terminal Phosphate Cyclase-like Protein, RNAC, RPC2, RTC2, HSPC338)
Product Gene Name
Product Synonym Names
Anti -RCL1, NT (RNA 3'-terminal Phosphate Cyclase-like Protein, RNAC, RPC2, RTC2, HSPC338)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human RCL1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
20,284 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (4792834..4861064). Location: 9p24.1-p23
3D Structure
ModBase 3D Structure for Q5VZU1
Immunogen
Synthetic peptide selected from the N-terminal region of human RCL1 (KLH).

Product Categories/Family for anti-RCL1 antibody

Applications Tested/Suitable for anti-RCL1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-RCL1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-RCL1 antibody

Western blot analysis of anti-RCL1 Pab in A375 cell line lysate (35ug/lane). RCL1(arrow) was detected using the purified Pab.
Rabbit Anti-RCL1 Polyclonal Antibody – Cat# MBS627927

Western Blot (WB) of anti-RCL1 antibody

Western blot analysis of RCL1 (arrow) using rabbit polyclonal RCL1 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCL1 gene.
Rabbit Anti-RCL1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS627927

Other Notes

Small volumes of anti-RCL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RCL1 purchase

MBS627927 is an antibody targeting RCL1. Also known as RCL1, NT (RNA 3'-terminal Phosphate Cyclase-like Protein, RNAC, RPC2, RTC2, HSPC338). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RCL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
RCL1
NCBI Official Synonym Full Names
RNA terminal phosphate cyclase-like 1
NCBI Official Symbol
RCL1
NCBI Official Synonym Symbols
RNAC; RPCL1
UniProt Protein Name
RCL1
UniProt Gene Name
RCL1
UniProt Entry Name
Q5VZU1_HUMAN
NCBI Protein Information
RNA 3'-terminal phosphate cyclase-like protein; 2310040A02Rik; OTTHUMP00000021017; RNA cyclase homolog

UniProt Comments for RCL1

RCL1: Does not have cyclase activity. Plays a role in 40S- ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 9p24.1-p23

Cellular Component: nucleolus

Molecular Function: endoribonuclease activity; RNA binding; RNA-3'-phosphate cyclase activity

Biological Process: RNA processing; endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-RCL1 antibody

Organs/Tissues associated with anti-RCL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with RCL1 and Liver
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