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Ribophorin (AT2G01720) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627995

Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an… More details

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Product Overview

Online Datasheet in PDF
Product Name
Ribophorin (AT2G01720), Polyclonal Antibody
Full Product Name
Ribophorin, CT (Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase 67kD Subunit, Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1, Ribophorin I, Ribophorin-1, RPN-I, RPN1)
Product Gene Name
Product Synonym Names
Anti -Ribophorin, CT (Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase 67kD Subunit, Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1, Ribophorin I, Ribophorin-1, RPN-I, RPN1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human RPN1. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
52,219 Da
Chromosome Location
Chromosome: 2; NC_003071.7 (317030..320264, complement). Location: chromosome: 2
3D Structure
ModBase 3D Structure for Q9ZUA0
Immunogen
Synthetic peptide selected from the C-terminal region of human RPN1 (KLH).
Applications Tested/Suitable for anti-AT2G01720 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-AT2G01720 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-AT2G01720 antibody

The anti-RPN1 Pab is used in Western blot to detect RPN1 in HeLa cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Rabbit Anti-AT2G01720 Polyclonal Antibody – Cat# MBS627995

Other Notes

Small volumes of anti-AT2G01720 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AT2G01720 purchase

MBS627995 is an antibody targeting AT2G01720. Also known as Ribophorin, CT (Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase 67kD Subunit, Dolichyl-diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1, Ribophorin I, Ribophorin-1, RPN-I, RPN1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AT2G01720. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ribophorin I
NCBI Official Symbol
AT2G01720
NCBI Official Synonym Symbols
T8O11.11; T8O11_11
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B
UniProt Synonym Protein Names
Ribophorin IB; RPN-IB; Ribophorin-1B
UniProt Gene Name
OST1B
UniProt Synonym Gene Names
RPN1B; RPN-IB
UniProt Entry Name
OST1B_ARATH
NCBI Protein Information
ribophorin I

UniProt Comments for AT2G01720

Function: Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains

By similarity.

Catalytic activity: Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathway: Protein modification; protein glycosylation.

Subunit structure: Component of the oligosaccharyltransferase (OST) complex

By similarity.

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein

By similarity.

Sequence similarities: Belongs to the OST1 family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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