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CNN1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627997

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CNN1, Polyclonal Antibody
Full Product Name
CNN1, NT (Calponin-1, Calponin H1, Smooth Muscle, Basic Calponin, Calponin 1)
Product Gene Name
Product Synonym Names
Anti -CNN1, NT (Calponin-1, Calponin H1, Smooth Muscle, Basic Calponin, Calponin 1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.39% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human Calponin-1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
33,170 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (11649579..11661138). Location: 19p13.2-p13.1
OMIM
600806
3D Structure
ModBase 3D Structure for P51911
Immunogen
Synthetic peptide selected from the N-terminal region of human Calponin-1 (KLH).
Applications Tested/Suitable for anti-CNN1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CNN1 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50

Western Blot (WB) of anti-CNN1 antibody

Western blot analysis of Calponin-1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.
Rabbit Anti-CNN1 Polyclonal Antibody – Cat# MBS627997

Immunohistochemistry (IHC) of anti-CNN1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CNN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627997

Flow Cytometry (FC/FACS) of anti-CNN1 antibody

Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CNN1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS627997

Other Notes

Small volumes of anti-CNN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CNN1 purchase

MBS627997 is an antibody targeting CNN1. Also known as CNN1, NT (Calponin-1, Calponin H1, Smooth Muscle, Basic Calponin, Calponin 1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CNN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calponin-1
NCBI Official Synonym Full Names
calponin 1, basic, smooth muscle
NCBI Official Symbol
CNN1
NCBI Official Synonym Symbols
SMCC; Sm-Calp
UniProt Protein Name
Calponin-1
UniProt Synonym Protein Names
Basic calponin; Calponin H1, smooth muscle
Protein Family
UniProt Gene Name
CNN1
UniProt Entry Name
CNN1_HUMAN
NCBI Protein Information
calponin-1; basic calponin; calponins, basic; calponin H1, smooth muscle

UniProt Comments for CNN1

calponin 1: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.

Protein type: Myosin-binding; Motility/polarity/chemotaxis; Actin-binding; Contractile

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: focal adhesion; cytoskeleton

Molecular Function: calmodulin binding; actin binding

Biological Process: regulation of smooth muscle contraction; actomyosin structure organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CNN1 antibody

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