Goat Anti-tif5A Polyclonal Antibody – Cat# MBS628058

MBS628058 (0.1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Eukaryotic Translation Initiation Factor 5a (tif5A) Antibody – Goat Polyclonal

Name: tif5A Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS628058
Price: 835 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628058

MBS628058 is an antibody targeting tif5A. Also known as Eukaryotic Translation Initiation Factor 5A (eIF 5A, eIF-5A, eIF5A Protein Synthesis Initiation Factor, Eukaryotic Initiation Factor 5A Isoform 1, Eukaryotic Translation Initiation Factor 5A-1, eIF 5A1,… More details

$835 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Eukaryotic Translation Initiation Factor 5A (tif5A), Polyclonal Antibody

Full Product Name

Eukaryotic Translation Initiation Factor 5A (eIF 5A, eIF-5A, eIF5A Protein Synthesis Initiation Factor, Eukaryotic Initiation Factor 5A Isoform 1, Eukaryotic Translation Initiation Factor 5A-1, eIF 5A1, eIF5A1, eIF-5A1, eIF-5A-1, eIF5AI, eIF 4D, eIF-4D, M

Product Gene Name

anti-tif5A antibody

Product Synonym Names

Anti -Eukaryotic Translation Initiation Factor 5A (eIF 5A, eIF-5A, eIF5A Protein Synthesis Initiation Factor, Eukaryotic Initiation Factor 5A Isoform 1, Eukaryotic Translation Initiation Factor 5A-1, eIF 5A1, eIF5A1, eIF-5A1, eIF-5A-1, eIF5AI, eIF 4D, eIF-4D, M

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Species Reactivity

Human

Form/Format

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purity/Purification

Affinity Purified
Purified by peptide affinity chromatography.

Specificity

Recognizes human eIF5A.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

17,497 Da

3D Structure

ModBase 3D Structure for P80639

Immunogen

Synthetic peptide corresponding to C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of human eIF5A (NP_001137232.1).

Product Categories/Family for anti-tif5A antibody

Antibodies; Abs to Translation Initiation Factors

Applications Tested/Suitable for anti-tif5A antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-tif5A antibody

Suitable for use in ELISA and Western Blot.
Dilution: Peptide ELISA Titer: 1:128,000
Western Blot: 1-3ug/ml detects a ~20kD band observed in MOLT4 lysates (calculated MW of 20.2kD according to NP_001137232.1).

Other Notes

Small volumes of anti-tif5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for tif5A. It may not necessarily be applicable to this product.

NCBI GI #

162458009

NCBI GeneID

542601

NCBI Accession #

NP_001105606.1

UniProt Primary Accession #

P80639

UniProt Secondary Accession #

P56332

UniProt Related Accession #

P80639

GenBank Nucleotide #

NM_001112136.1

NCBI Official Full Name

eukaryotic translation initiation factor 5A

NCBI Official Synonym Full Names

eukaryotic translation initiation factor 5A

NCBI Official Symbol

tif5A

UniProt Protein Name

Eukaryotic translation initiation factor 5A

UniProt Synonym Protein Names

eIF-4D

Protein Family

Eukaryotic translation initiation factor

UniProt Gene Name

TIF5A

UniProt Entry Name

IF5A_MAIZE

NCBI Protein Information

eukaryotic translation initiation factor 5A; csu154; csu175; csu203; eIF-4D; eIF-5A; SOG0075; csu154b; umc384c; S1_eIF5A; csu154c(eif5A); csu175d(eif5A); csu203a(eif5A)

UniProt Comments for tif5A

Function: The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.

Post-translational modification: eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Sequence similarities: Belongs to the eIF-5A family.

Research Articles on tif5A

1. phosphorylation of Ser(2) plays a role in regulation of nucleocytoplasmic shuttling of eIF5A in plant cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.