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ACO2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628098

ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion.… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ACO2, Polyclonal Antibody
Full Product Name
ACO2, ID (Aconitate Hydratase, Mitochondrial, Citrate Hydro-lyase, Aconitase)
Product Gene Name
Product Synonym Names
Anti -ACO2, ID (Aconitate Hydratase, Mitochondrial, Citrate Hydro-lyase, Aconitase)
Clonality
Polyclonal
Isotype
IgG
Host
rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
85,425 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (41865129..41924993). Location: 22q11.2-q13.31; 22q13.2
OMIM
100850
3D Structure
ModBase 3D Structure for Q99798
Immunogen
Synthetic peptide selected from the central region of human Aconitase (KLH).

Product Categories/Family for anti-ACO2 antibody

Applications Tested/Suitable for anti-ACO2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ACO2 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:10-1:50

Western Blot (WB) of anti-ACO2 antibody

Western blot analysis of anti-ACO2 Pab in mouse heart(left) and 293 (right) tissue lysates (35ug/lane). ACO2(arrow) was detected using the purified Pab.
Rabbit Anti-ACO2 Polyclonal Antibody – Cat# MBS628098

Testing Data of anti-ACO2 antibody

Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center) . Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
Rabbit Anti-ACO2 Polyclonal Antibody, Testing Data – Cat# MBS628098

Western Blot (WB) of anti-ACO2 antibody

ACO2 western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).
Rabbit Anti-ACO2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS628098

Immunohistochemistry (IHC) of anti-ACO2 antibody

Formalin-fixed and paraffin-embedded human Heart tissue reacted with ACO2 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ACO2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628098

Other Notes

Small volumes of anti-ACO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACO2 purchase

MBS628098 is an antibody targeting ACO2. Also known as ACO2, ID (Aconitate Hydratase, Mitochondrial, Citrate Hydro-lyase, Aconitase). Reactive in Human, Mouse, Rat. Host: rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACO2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
ACO2
NCBI Official Synonym Full Names
aconitase 2, mitochondrial
NCBI Official Symbol
ACO2
NCBI Official Synonym Symbols
ACONM; MGC20605; MGC33908
UniProt Protein Name
Aconitate hydratase, mitochondrial
UniProt Synonym Protein Names
Citrate hydro-lyase
UniProt Gene Name
ACO2
UniProt Entry Name
ACON_HUMAN
NCBI Protein Information
aconitate hydratase, mitochondrial; OTTHUMP00000165920; OTTHUMP00000165921; citrate hydro-lyase

NCBI Summary for ACO2

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]
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UniProt Comments for ACO2

ACO2: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. Monomer. Belongs to the aconitase/IPM isomerase family.

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.2.1.3; Lyase; Mitochondrial

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: mitochondrion; mitochondrial matrix; nucleus

Molecular Function: iron ion binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; 3 iron, 4 sulfur cluster binding

Biological Process: isocitrate metabolic process; cellular metabolic process; generation of precursor metabolites and energy; tricarboxylic acid cycle; citrate metabolic process

Disease: Infantile Cerebellar-retinal Degeneration; Optic Atrophy 8
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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