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TUFM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628403

TUFM is a protein which participates in protein translation in mitochondria. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TUFM, Polyclonal Antibody
Full Product Name
TUFM, NT (Elongation Factor Tu, Mitochondrial, EF-Tu, P43)
Product Gene Name
anti-Tufm antibody
Product Synonym Names
Anti -TUFM, NT (Elongation Factor Tu, Mitochondrial, EF-Tu, P43)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.104% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human TUFM.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,508 Da
Chromosome Location
Chromosome: 7; NC_000073.5 (133630869..133634245). Location: 7 F3; 7
3D Structure
ModBase 3D Structure for Q8BFR5
Immunogen
Synthetic peptide selected from the N-terminal region of human TUFM (KLH).

Product Categories/Family for anti-Tufm antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-Tufm antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Tufm antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-Tufm antibody

Western blot analysis of TUFM Antibody (N-term) in Ramos, Hela cell line lysates (35ug/lane). TUFM (arrow) was detected using the purified Pab.
Rabbit Anti-TUFM Polyclonal Antibody – Cat# MBS628403

Immunohistochemistry (IHC) of anti-Tufm antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with TUFM Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-TUFM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628403

Flow Cytometry (FC/FACS) of anti-Tufm antibody

TUFM Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-TUFM Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS628403

Western Blot (WB) of anti-Tufm antibody

Western blot analysis of TUFM (arrow) using rabbit polyclonal TUFM Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TUFM gene.
Rabbit Anti-TUFM Polyclonal Antibody, Western Blot (WB) – Cat# MBS628403

Other Notes

Small volumes of anti-Tufm antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tufm purchase

MBS628403 is an antibody targeting Tufm. Also known as TUFM, NT (Elongation Factor Tu, Mitochondrial, EF-Tu, P43). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Tufm. It may not necessarily be applicable to this product.

NCBI GI #
38173913
NCBI GeneID
233870
UniProt Primary Accession #
Q8BFR5
UniProt Secondary Accession #
Q497E7; Q6P919
UniProt Related Accession #
Q8BFR5
NCBI Official Full Name
Tufm protein
NCBI Official Synonym Full Names
Tu translation elongation factor, mitochondrial
NCBI Official Symbol
Tufm
NCBI Official Synonym Symbols
EFTU; C76308; C76389; EF-TuMT; 2300002G02Rik
UniProt Protein Name
Elongation factor Tu, mitochondrial
UniProt Gene Name
Tufm
UniProt Entry Name
EFTU_MOUSE
NCBI Protein Information
elongation factor Tu, mitochondrial

UniProt Comments for Tufm

EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Protein type: Translation elongation; Mitochondrial; Translation; RNA-binding

Cellular Component: membrane; mitochondrion; mitochondrial inner membrane; intracellular; myelin sheath

Molecular Function: GTPase activity; GTP binding; nucleotide binding; translation elongation factor activity

Biological Process: translational elongation; translation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-Tufm antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with Tufm and Neoplasms
 Breast Neoplasms Antibodies  >5 publications with Tufm and Breast Neoplasms
 Carcinoma Antibodies  >4 publications with Tufm and Carcinoma
 Adenocarcinoma Antibodies  >3 publications with Tufm and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >2 publications with Tufm and Cell Transformation, Neoplastic
 Muscular Diseases Antibodies  >2 publications with Tufm and Muscular Diseases
 Mitochondrial Diseases Antibodies  >2 publications with Tufm and Mitochondrial Diseases
 Inflammation Antibodies  >2 publications with Tufm and Inflammation
 Leukemia Antibodies  >1 publications with Tufm and Leukemia
 Lung Neoplasms Antibodies  >1 publications with Tufm and Lung Neoplasms

Organs/Tissues associated with anti-Tufm antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with Tufm and Blood
 Brain Antibodies  >4 publications with Tufm and Brain
 Liver Antibodies  >3 publications with Tufm and Liver
 Spleen Antibodies  >2 publications with Tufm and Spleen
 Eye Antibodies  >2 publications with Tufm and Eye
 Lung Antibodies  >2 publications with Tufm and Lung
 Pituitary Gland Antibodies  >1 publications with Tufm and Pituitary Gland
 Stomach Antibodies  >1 publications with Tufm and Stomach
 Heart Antibodies  >1 publications with Tufm and Heart
 Kidney Antibodies  >1 publications with Tufm and Kidney
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