Rabbit Anti-SOAT1 Polyclonal Antibody – Cat# MBS628431

Peptide Competition Extracts prepared from HEK293 cells left untreated (1) or treated with UV (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with STAT1 [pS727] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRPconjugate and bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to STAT1 [pS727] blocks the antibody signal, demonstrating the specificity of the antibody.

STAT 1 (SOAT1) Antibody – Rabbit Polyclonal

Name: SOAT1 Antibody; Polyclonal; Rabbit; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS628431
Price: 1000 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628431

The signal transducer and activator of transcription (STAT) family of transcription factors is composed of seven family members (STATs 1, 2, 3, 4, 5A, 5B and 6) that are involved in many cell processes (apoptosis, anti-apoptosis, cell differentiation and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

STAT 1 (SOAT1), Polyclonal Antibody

Full Product Name

STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1)

Product Gene Name

anti-SOAT1 antibody

Product Synonym Names

Anti -STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse

Form/Format

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human and mouse STAT1 when phosphorylated at Ser727.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

64,735 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (179262849..179327815). Location: 1q25

OMIM

102642

3D Structure

ModBase 3D Structure for P35610

Immunogen

Synthetic phosphopeptide corresponding to human STAT1 that contains serine 727.

Positive Control

HEK293 cells treated with UV light; 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).

Product Categories/Family for anti-SOAT1 antibody

Antibodies; Abs to Transcription Factors; STAT

Applications Tested/Suitable for anti-SOAT1 antibody

Western Blot (WB)

Application Notes for anti-SOAT1 antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Optimal dilution to be determined by the researcher.

Other Notes

Small volumes of anti-SOAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOAT1 purchase

MBS628431 is an antibody targeting SOAT1. Also known as STAT 1, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SOAT1. It may not necessarily be applicable to this product.

NCBI GI #

49533617

NCBI GeneID

6646

NCBI Accession #

NP_003092.4

UniProt Primary Accession #

P35610

UniProt Secondary Accession #

Q5T0X4; Q8N1E4; A6NC40; A8K3P4; A9Z1V7; B4DU95

UniProt Related Accession #

P35610

GenBank Nucleotide #

NM_003101.5

NCBI Official Full Name

sterol O-acyltransferase 1 isoform 1

NCBI Official Synonym Full Names

sterol O-acyltransferase 1

NCBI Official Symbol

SOAT1

NCBI Official Synonym Symbols

ACAT; SOAT; STAT; ACACT; ACAT1; ACAT-1

UniProt Protein Name

Sterol O-acyltransferase 1

UniProt Synonym Protein Names

Acyl-coenzyme A:cholesterol acyltransferase 1; ACAT-1; Cholesterol acyltransferase 1

Protein Family

Sterol O-acyltransferase

UniProt Gene Name

SOAT1

UniProt Synonym Gene Names

ACACT; ACACT1; ACAT; ACAT1; SOAT; STAT; ACAT-1

UniProt Entry Name

SOAT1_HUMAN

NCBI Protein Information

sterol O-acyltransferase 1; acyl-coenzyme A:cholesterol acyltransferase 1; sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1

NCBI Summary for SOAT1

The protein encoded by this gene belongs to the acyltransferase family. It is located in the endoplasmic reticulum, and catalyzes the formation of fatty acid-cholesterol esters. This gene has been implicated in the formation of beta-amyloid and atherosclerotic plaques by controlling the equilibrium between free cholesterol and cytoplasmic cholesteryl esters. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011]

UniProt Comments for SOAT1

SOAT1: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Protein type: Transferase; Membrane protein, integral; EC 2.3.1.26; Membrane protein, multi-pass; Lipid Metabolism - steroid biosynthesis

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; acyl-CoA binding; sterol O-acyltransferase activity; cholesterol binding

Biological Process: cholesterol metabolic process; cholesterol homeostasis; cholesterol efflux; positive regulation of amyloid precursor protein biosynthetic process

Research Articles on SOAT1

1. These studies demonstrate that both SIAE and SOAT activities seem to be responsible for the enhanced level of Neu5,9Ac(2) in lymphoblasts, which is a hallmark in acute lymphoblastic leukemia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SOAT1 antibody

Reference Product	PubMed Publications
CYP46A1 antibody	>2 publications with SOAT1 and CYP46A1
LIPA antibody	>2 publications with SOAT1 and LIPA
CYP27A1 antibody	>1 publications with SOAT1 and CYP27A1
CH25H antibody	>1 publications with SOAT1 and CH25H
CYP7A1 antibody	>1 publications with SOAT1 and CYP7A1
DHCR24 antibody	>1 publications with SOAT1 and DHCR24

Pathways associated with anti-SOAT1 antibody

Products by Pathway	Pathway Diagram
Statin Pathway antibodies	Statin Pathway Diagram
Steroid Biosynthesis Pathway antibodies	Steroid Biosynthesis Pathway Diagram
Steroid Biosynthesis Pathway antibodies	Steroid Biosynthesis Pathway Diagram

Diseases associated with anti-SOAT1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with SOAT1 and Inflammation
Myocardial Infarction Antibodies	>1 publications with SOAT1 and Myocardial Infarction
Neoplasms Antibodies	>1 publications with SOAT1 and Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with SOAT1 and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>1 publications with SOAT1 and Liver Diseases
Fatty Liver Antibodies	>1 publications with SOAT1 and Fatty Liver
Liver Neoplasms Antibodies	>1 publications with SOAT1 and Liver Neoplasms

Organs/Tissues associated with anti-SOAT1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>6 publications with SOAT1 and Brain
Eye Antibodies	>2 publications with SOAT1 and Eye
Vascular Antibodies	>2 publications with SOAT1 and Vascular
Liver Antibodies	>2 publications with SOAT1 and Liver
Blood Antibodies	>2 publications with SOAT1 and Blood
Heart Antibodies	>1 publications with SOAT1 and Heart
Bladder Antibodies	>1 publications with SOAT1 and Bladder
Adrenal Gland Antibodies	>1 publications with SOAT1 and Adrenal Gland