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Caspase 3, Cleaved Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628492

Caspase 3 is one of the key executioners of apoptosis. It is responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase 3 requires proteolytic… More details

$830 / 0.1 mL +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Caspase 3, Cleaved, Polyclonal Antibody
Full Product Name
Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Product Synonym Names
Anti -Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Specificity
Recognizes cleaved human Caspase 3 (Asp175). Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Detects non-specific caspase sunstrates by Western Blot.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues adjacent to Asp175 in N-terminus of human caspase-3.
Species Sequence Homology
Porcine, bovine and canine.
Applications Tested/Suitable for anti-Caspase 3, Cleaved antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Caspase 3, Cleaved antibody
Dilution: Immunofluorescence: 1:400
Flow Cytometry: 1:800
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:300
Immunohistochemistry (frozen): 1:600 fixed in 3% Fromaldehyde.
Immunofluorescence (IC): 1:400
Immunoprecipitation: 1:100

Western Blot (WB) of anti-Caspase 3, Cleaved antibody

Western Blot: Extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (1uM in vivo) or cytochrome c-treated (0.25mg/ml in vitro), using C2087-16 (upper) or MBS628492 (lower).
Rabbit Anti-Caspase 3, Cleaved Polyclonal Antibody – Cat# MBS628492

Immunohistochemistry (IHC) of anti-Caspase 3, Cleaved antibody

Immunohistochemical analysis of paraffin-embedded mouse embryo, using MBS628492 preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (right).
Rabbit Anti-Caspase 3, Cleaved Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628492

Immunohistochemistry (IHC) of anti-Caspase 3, Cleaved antibody

Immunohistochemical analysis of frozen H1650 xenograft section, using MBS628492.
Rabbit Anti-Caspase 3, Cleaved Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628492

Immunohistochemistry (IHC) of anti-Caspase 3, Cleaved antibody

Immunohistochemical analysis of paraffin-embedded Jurkat cells, untreated (left) or etoposide treated (right), using MBS628492.
Rabbit Anti-Caspase 3, Cleaved Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628492

Immunofluorescence (IF) of anti-Caspase 3, Cleaved antibody

Confocal IF images of HT-29 cells, untreated (L) or staurosporine-treated (R), labeled with MBS628492 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Rabbit Anti-Caspase 3, Cleaved Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS628492

Flow Cytometry (FC/FACS) of anti-Caspase 3, Cleaved antibody

Flow cytometric analysis of Pam212 cells, untreated or sulindac sulfone- treated (to induce apoptosis), using MBS628492 Results were similar to those obtained by analyzing DNA content.
Rabbit Anti-Caspase 3, Cleaved Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS628492

Other Notes

Small volumes of anti-Caspase 3, Cleaved antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Caspase 3, Cleaved purchase

MBS628492 is an antibody targeting Caspase 3, Cleaved. Also known as Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Caspase 3, Cleaved. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
caspase 3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Caspase 3, Cleaved antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Caspase 3, Cleaved and FLNA
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