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CFL2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6285382

MBS6285382 is an antibody targeting CFL2. Also known as CFL2, NT (CFL2, Cofilin-2, Cofilin, muscle isoform) (APC). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CFL2, Polyclonal Antibody
Full Product Name
CFL2, NT (CFL2, Cofilin-2, Cofilin, muscle isoform) (APC)
Product Gene Name
anti-CFL2 antibody
Product Synonym Names
CFL2; Cofilin-2; Cofilin; muscle isoform
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
– Da
OMIM
601443
3D Structure
ModBase 3D Structure for Q9Y281
Immunogen
CFL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human CFL2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-CFL2 antibody

Antibodies; Actin Related Proteins
Applications Tested/Suitable for anti-CFL2 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-CFL2 antibody
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CFL2 antibody

Western blot analysis of CFL2 (arrow) using rabbit polyclonal CFL2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CFL2 gene.
Rabbit Anti-CFL2 Polyclonal Antibody – Cat# MBS6285382

Western Blot (WB) of anti-CFL2 antibody

CFL2 Antibody (N-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the CFL2 antibody detected the CFL2 protein (arrow).
Rabbit Anti-CFL2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6285382

Immunohistochemistry (IHC) of anti-CFL2 antibody

CFL2 Antibody (N-term) (AP14117a)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CFL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CFL2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6285382

Product References and Citations for anti-CFL2 antibody

Vogel, S., et al. J. Biol. Chem. 285(44):33756-33763(2010). Borensztajn, K., et al. Thromb. Res. 125 (6), E323-E328 (2010): Papalouka, V., et al. Mol. Cell. Biol. 29(22):6046-6058(2009). Wu, Y., et al. Retrovirology 5, 95 (2008): Agrawal, P.B., et al. Am. J. Hum. Genet. 80(1):162-167(2007).

Other Notes

Small volumes of anti-CFL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CFL2. It may not necessarily be applicable to this product.

NCBI GI #
33946278
NCBI GeneID
1073
NCBI Accession #
NP_619579.1
NCBI Related Accession #
NP_068733.1
UniProt Primary Accession #
Q9Y281
UniProt Secondary Accession #
G3V5P4
UniProt Related Accession #
Q9Y281
GenBank Nucleotide #
NM_138638.4
NCBI Official Full Name
cofilin-2 isoform 1
NCBI Official Synonym Full Names
cofilin 2 (muscle)
NCBI Official Symbol
CFL2
NCBI Official Synonym Symbols
NEM7
UniProt Protein Name
Cofilin-2
UniProt Synonym Protein Names
Cofilin, muscle isoform
Protein Family
Cofilin
UniProt Gene Name
CFL2
UniProt Entry Name
COF2_HUMAN
NCBI Protein Information
cofilin-2; cofilin, muscle isoform; nemaline myopathy type 7

NCBI Summary for CFL2

This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
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UniProt Comments for CFL2

Cofilin-2: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: I band; extracellular space; nuclear matrix; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: muscle maintenance; actin filament depolymerization; positive regulation of actin filament depolymerization; sarcomere organization

Disease: Nemaline Myopathy 7
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Research Articles on CFL2

1. patients with severe nemaline myopathy should be screened for mutations in CFL2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CFL2 antibody

 Reference Product  PubMed Publications
 LIMK1 antibody  >3 publications with CFL2 and LIMK1
 RHOA antibody  >1 publications with CFL2 and RHOA
 LIMK2 antibody  >1 publications with CFL2 and LIMK2

Pathways associated with anti-CFL2 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 G13 Signaling Pathway antibodies  G13 Signaling Pathway Diagram
 Leptin Signaling Pathway antibodies  Leptin Signaling Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram

Diseases associated with anti-CFL2 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with CFL2 and Disease Models, Animal
 Nemaline Myopathy 7 Antibodies  >2 publications with CFL2 and Nemaline Myopathy 7
 Nervous System Malformations Antibodies  >1 publications with CFL2 and Nervous System Malformations
 Neoplasm Metastasis Antibodies  >1 publications with CFL2 and Neoplasm Metastasis
 Carcinoma Antibodies  >1 publications with CFL2 and Carcinoma
 Neural Tube Defects Antibodies  >1 publications with CFL2 and Neural Tube Defects
 Adenocarcinoma Antibodies  >1 publications with CFL2 and Adenocarcinoma
 Hyperplasia Antibodies  >1 publications with CFL2 and Hyperplasia

Organs/Tissues associated with anti-CFL2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >23 publications with CFL2 and Muscle
 Vascular Antibodies  >4 publications with CFL2 and Vascular
 Heart Antibodies  >4 publications with CFL2 and Heart
 Blood Antibodies  >3 publications with CFL2 and Blood
 Embryonic Tissue Antibodies  >2 publications with CFL2 and Embryonic Tissue
 Adipose Tissue Antibodies  >1 publications with CFL2 and Adipose Tissue
 Connective Tissue Antibodies  >1 publications with CFL2 and Connective Tissue
 Testis Antibodies  >1 publications with CFL2 and Testis
 Kidney Antibodies  >1 publications with CFL2 and Kidney
 Brain Antibodies  >1 publications with CFL2 and Brain
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