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Transglutaminase Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628545

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Transglutaminase, Polyclonal Antibody
Full Product Name
Transglutaminase, ID (K444) (Protein-glutamine gamma-glutamyltransferase 2, Tissue Transglutaminase, Transglutaminase C, TGase C, TGC, TG(C), Transglutaminase-2, TGase-2, Transglutaminase H, TGase-H, TGM2)
Product Synonym Names
Anti -Transglutaminase, ID (K444) (Protein-glutamine gamma-glutamyltransferase 2, Tissue Transglutaminase, Transglutaminase C, TGase C, TGC, TG(C), Transglutaminase-2, TGase-2, Transglutaminase H, TGase-H, TGM2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TGM2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the Center region of human TGM2 (KLH).
Applications Tested/Suitable for anti-Transglutaminase antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Transglutaminase antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50

Western Blot (WB) of anti-Transglutaminase antibody

TGM2 Antibody (Center K444) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).
Rabbit Anti-Transglutaminase Polyclonal Antibody – Cat# MBS628545

Immunofluorescence (IF) of anti-Transglutaminase antibody

Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444) .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.
Rabbit Anti-Transglutaminase Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS628545

Immunohistochemistry (IHC) of anti-Transglutaminase antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-Transglutaminase Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628545

Other Notes

Small volumes of anti-Transglutaminase antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Transglutaminase purchase

MBS628545 is an antibody targeting Transglutaminase. Also known as Transglutaminase, ID (K444) (Protein-glutamine gamma-glutamyltransferase 2, Tissue Transglutaminase, Transglutaminase C, TGase C, TGC, TG(C), Transglutaminase-2, TGase-2, Transglutaminase H, TGase-H, TGM2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Transglutaminase. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
transglutaminase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Transglutaminase antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Transglutaminase and FLNA
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