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Histidyl-Trna Synthetase (hisS) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628654

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of… More details

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Product Overview

Online Datasheet in PDF
Product Name
Histidyl-tRNA Synthetase (hisS), Polyclonal Antibody
Full Product Name
Histidyl-tRNA Synthetase, NT (HRS, HARS, HisRS, Histidine-tRNA Ligase, FLJ20491)
Product Gene Name
Product Synonym Names
Anti -Histidyl-tRNA Synthetase, NT (HRS, HARS, HisRS, Histidine-tRNA Ligase, FLJ20491)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human HARS.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,029 Da
Chromosome Location
NC_000913.2 (2637323..2638597, complement)
3D Structure
ModBase 3D Structure for P60906
Immunogen
Synthetic peptide selected from the N-terminal region of human HARS (KLH).
Applications Tested/Suitable for anti-hisS antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-hisS antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:10-1:50

Western Blot (WB) of anti-hisS antibody

Western blot analysis of anti-HARS Pab in K562 cell line lysates (35ug/lane).HARS (arrow) was detected using the purified Pab.
Rabbit Anti-hisS Polyclonal Antibody – Cat# MBS628654

Immunohistochemistry (IHC) of anti-hisS antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-hisS Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628654

Other Notes

Small volumes of anti-hisS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for hisS purchase

MBS628654 is an antibody targeting hisS. Also known as Histidyl-tRNA Synthetase, NT (HRS, HARS, HisRS, Histidine-tRNA Ligase, FLJ20491). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for hisS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
histidyl tRNA synthetase
NCBI Official Symbol
hisS
NCBI Official Synonym Symbols
ECK2510; JW2498
UniProt Protein Name
Histidyl-tRNA synthetase
UniProt Synonym Protein Names
Histidine--tRNA ligase
UniProt Gene Name
hisS
UniProt Entry Name
SYH_ECOLI
NCBI Protein Information
histidyl tRNA synthetase

NCBI Summary for hisS

Histidyl-tRNA synthetase (HisRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG10453].

UniProt Comments for hisS

Catalytic activity: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP MF_00127

Subunit structure: Homodimer.

Subcellular location: Cytoplasm HAMAP MF_00127.

Sequence similarities: Belongs to the class-II aminoacyl-tRNA synthetase family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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