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CDC25A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628707

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to… More details

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Product Overview

Online Datasheet in PDF
Product Name
CDC25A, Polyclonal Antibody
Full Product Name
CDC25A, phosphorylated (Ser278) (M-phase Inducer Phosphatase 1, Dual Specificity Phosphatase Cdc25A)
Product Synonym Names
Anti -CDC25A, phosphorylated (Ser278) (M-phase Inducer Phosphatase 1, Dual Specificity Phosphatase Cdc25A)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Cdc25A phosphorylated at Ser278.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser278 of human Cdc25A (KLH).

Product Categories/Family for anti-CDC25A antibody

Antibodies; Abs to Enzymes; Phosphatase
Applications Tested/Suitable for anti-CDC25A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CDC25A antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-CDC25A antibody

The anti-Phospho-CDC25A-S278 Pab is used in Western blot to detect Phospho-CDC25A-S278 in mouse heart tissue lysate
Rabbit Anti-CDC25A Polyclonal Antibody – Cat# MBS628707

Immunohistochemistry (IHC) of anti-CDC25A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-CDC25A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628707

Other Notes

Small volumes of anti-CDC25A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDC25A purchase

MBS628707 is an antibody targeting CDC25A. Also known as CDC25A, phosphorylated (Ser278) (M-phase Inducer Phosphatase 1, Dual Specificity Phosphatase Cdc25A). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC25A. It may not necessarily be applicable to this product.

NCBI GI #
68135376
NCBI Official Full Name
CDC25A
Protein Family
M-phase inducer phosphatase

UniProt Comments for CDC25A

CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described.

Protein type: Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; response to radiation; cell division; regulation of cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDC25A antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with CDC25A and FLNA

Pathways associated with anti-CDC25A antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Organs/Tissues associated with anti-CDC25A antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with CDC25A and Bone
 Connective Tissue Antibodies  >4 publications with CDC25A and Connective Tissue
 Heart Antibodies  >3 publications with CDC25A and Heart
 Muscle Antibodies  >3 publications with CDC25A and Muscle
 Brain Antibodies  >3 publications with CDC25A and Brain
 Vascular Antibodies  >2 publications with CDC25A and Vascular
 Embryonic Tissue Antibodies  >2 publications with CDC25A and Embryonic Tissue
 Ear Antibodies  >1 publications with CDC25A and Ear
 Kidney Antibodies  >1 publications with CDC25A and Kidney
 Umbilical Cord Antibodies  >1 publications with CDC25A and Umbilical Cord
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