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UBE4B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628714

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s,… More details

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Product Overview

Online Datasheet in PDF
Product Name
UBE4B, Polyclonal Antibody
Full Product Name
UBE4B, NT (Ubiquitin Conjugation Factor E4 B, Ubiquitin-fusion Degradation Protein 2, Homozygously Deleted In Neuroblastoma-1, HDNB1, KIAA0684, UFD2)
Product Gene Name
Product Synonym Names
Anti -UBE4B, NT (Ubiquitin Conjugation Factor E4 B, Ubiquitin-fusion Degradation Protein 2, Homozygously Deleted In Neuroblastoma-1, HDNB1, KIAA0684, UFD2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human UBE4B. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
146,185 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (10093016..10241297). Location: 1p36.3
OMIM
613565
3D Structure
ModBase 3D Structure for O95155
Immunogen
Synthetic peptide selected from the N-terminal region of human UBE4B (KLH).

Product Categories/Family for anti-UBE4B antibody

Applications Tested/Suitable for anti-UBE4B antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-UBE4B antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-UBE4B antibody

The anti-UBE4B Pab is used in Western blot to detect UBE4B in mouse kidney tissue lysate.
Rabbit Anti-UBE4B Polyclonal Antibody – Cat# MBS628714

Immunohistochemistry (IHC) of anti-UBE4B antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-UBE4B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628714

Other Notes

Small volumes of anti-UBE4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE4B purchase

MBS628714 is an antibody targeting UBE4B. Also known as UBE4B, NT (Ubiquitin Conjugation Factor E4 B, Ubiquitin-fusion Degradation Protein 2, Homozygously Deleted In Neuroblastoma-1, HDNB1, KIAA0684, UFD2). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE4B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin conjugation factor E4 B isoform 2
NCBI Official Synonym Full Names
ubiquitination factor E4B
NCBI Official Symbol
UBE4B
NCBI Official Synonym Symbols
E4; UFD2; HDNB1; UBOX3; UFD2A; KIAA0684
UniProt Protein Name
Ubiquitin conjugation factor E4 B
UniProt Synonym Protein Names
Homozygously deleted in neuroblastoma 1; Ubiquitin fusion degradation protein 2
UniProt Gene Name
UBE4B
UniProt Synonym Gene Names
HDNB1; KIAA0684; UFD2
UniProt Entry Name
UBE4B_HUMAN
NCBI Protein Information
ubiquitin conjugation factor E4 B; OTTHUMP00000001726; OTTHUMP00000001727; homologous to yeast UFD2; UFD2A-III/UBE4B-III splice isoform; ubiquitin fusion degradation protein 2; ubiquitin-fusion degradation protein 2; homozygously deleted in neuroblastoma 1; homozygously deleted in neuroblastoma-1; ubiquitination factor E4B (UFD2 homolog, yeast); ubiquitination factor E4B (homologous to yeast UFD2)

NCBI Summary for UBE4B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
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UniProt Comments for UBE4B

UBE4B: Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. Belongs to the ubiquitin conjugation factor E4 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: enzyme binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; induction of apoptosis by granzyme; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to UV
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UBE4B antibody

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