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IGJ Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628814

IGJ serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory… More details

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Product Overview

Online Datasheet in PDF
Product Name
IGJ, Polyclonal Antibody
Full Product Name
IGJ, NT (Immunoglobulin J Chain, IGCJ, Immunoglobulin J Polypeptide Linker Protein for Immunoglobulin alpha and mu Polypeptides, JCH)
Product Gene Name
Product Synonym Names
Anti -IGJ, NT (Immunoglobulin J Chain, IGCJ, Immunoglobulin J Polypeptide Linker Protein for Immunoglobulin alpha and mu Polypeptides, JCH)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human IGJ.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
18,099 Da
Chromosome Location
Chromosome: 4; NC_000004.11 (71521258..71532348, complement). Location: 4q21
OMIM
147790
3D Structure
ModBase 3D Structure for P01591
Immunogen
Synthetic peptide selected from the N-terminal region of human IGJ (KLH).
Applications Tested/Suitable for anti-IGJ antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-IGJ antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-IGJ antibody

Western Blot analysis of mouse spleen lysates (35ug/lane) using MBS628814. IGJ (arrow) was detected using MBS628814.
Rabbit Anti-IGJ Polyclonal Antibody – Cat# MBS628814

Immunohistochemistry (IHC) of anti-IGJ antibody

Formalin-fixed and paraffin-embedded mouse spleen reacted with MBS628814 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS628814 for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-IGJ Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS628814

Flow Cytometry (FC/FACS) of anti-IGJ antibody

Flow cytometric analysis of HL-60 cells (right histogram) using MBS628814 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-IGJ Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS628814

Other Notes

Small volumes of anti-IGJ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IGJ purchase

MBS628814 is an antibody targeting IGJ. Also known as IGJ, NT (Immunoglobulin J Chain, IGCJ, Immunoglobulin J Polypeptide Linker Protein for Immunoglobulin alpha and mu Polypeptides, JCH). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IGJ. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
immunoglobulin J chain
NCBI Official Synonym Full Names
immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
NCBI Official Symbol
IGJ
NCBI Official Synonym Symbols
JCH; IGCJ
UniProt Protein Name
Immunoglobulin J chain
UniProt Gene Name
IGJ
UniProt Synonym Gene Names
IGCJ
UniProt Entry Name
IGJ_HUMAN
NCBI Protein Information
immunoglobulin J chain; IgJ chain

UniProt Comments for IGJ

IGJ: Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidoglycan binding; protein homodimerization activity; IgA binding; phosphatidylcholine binding; antigen binding; single-stranded DNA binding

Biological Process: retinal homeostasis; positive regulation of protein oligomerization; adaptive immune response; glomerular filtration; antibacterial humoral response; innate immune response; immune response
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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