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Reptin (rept) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS628914

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and… More details

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Product Overview

Online Datasheet in PDF
Product Name
Reptin, Polyclonal Antibody
Full Product Name
Reptin, CT (RuvB-like 2, 48kD TATA Box-binding Protein-interacting Protein, 48kD TBP-Interacting Protein, TIP49b, Repressing Pontin 52, Reptin 52, 51kD Erythrocyte Cytosolic Protein, ECP-51, TIP60-associated Protein 54-beta, TAP54-beta, TIP48, TIP49B, RUV
Product Gene Name
anti-rept antibody
Product Synonym Names
Anti -Reptin, CT (RuvB-like 2, 48kD TATA Box-binding Protein-interacting Protein, 48kD TBP-Interacting Protein, TIP49b, Repressing Pontin 52, Reptin 52, 51kD Erythrocyte Cytosolic Protein, ECP-51, TIP60-associated Protein 54-beta, TAP54-beta, TIP48, TIP49B, RUV
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human RUVBL2. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
53,541 Da
Chromosome Location
Chromosome: 3L; NT_037436.3 (19248440..19250211). Location: 76A3-76A3
3D Structure
ModBase 3D Structure for Q9V3K3
Immunogen
Synthetic peptide selected from the C-terminal region of human RUVBL2 (KLH).

Product Categories/Family for anti-rept antibody

Antibodies; Abs to Enzymes
Applications Tested/Suitable for anti-rept antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-rept antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:250-1:500

Western Blot (WB) of anti-rept antibody

Western Blot analysis detects RUVBL2 in mouse kidney (left) and SK-Br-3 (right) tissue/cell linel ysates using MBS628914.
Rabbit Anti-rept Polyclonal Antibody – Cat# MBS628914

Western Blot (WB) of anti-rept antibody

Western Blot analysis of RUVBL2 (arrow) of 293 cell lysates (2ug/lane) using MBS628914. Lane 1: Nontransfected with the RUVBL2 gene. Lane 2: Transfected with the RUVBL2 gene
Rabbit Anti-rept Polyclonal Antibody, Western Blot (WB) – Cat# MBS628914

Other Notes

Small volumes of anti-rept antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rept purchase

MBS628914 is an antibody targeting rept. Also known as Reptin, CT (RuvB-like 2, 48kD TATA Box-binding Protein-interacting Protein, 48kD TBP-Interacting Protein, TIP49b, Repressing Pontin 52, Reptin 52, 51kD Erythrocyte Cytosolic Protein, ECP-51, TIP60-associated Protein 54-beta, TAP54-beta, TIP48, TIP49B, RUV. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for rept. It may not necessarily be applicable to this product.

NCBI GI #
442633310
NCBI GeneID
40092
NCBI Accession #
NP_001262040.1
UniProt Primary Accession #
Q9V3K3
UniProt Related Accession #
Q9V3K3
GenBank Nucleotide #
NM_001275111.1
NCBI Official Full Name
reptin, isoform B
NCBI Official Synonym Full Names
reptin
NCBI Official Symbol
rept
NCBI Official Synonym Symbols
anon-WO0142479.1; CG9750; DmelCG9750; drep; dRep; dReptin; l(3)06945; rep; Rept; REPT; Reptin; Tip48; Tip48/Reptin
UniProt Protein Name
RuvB-like helicase 2
UniProt Synonym Protein Names
Dreptin; Drep; Reptin
UniProt Gene Name
rept
UniProt Synonym Gene Names
Drep
UniProt Entry Name
RUVB2_DROME
NCBI Protein Information
CG9750 gene product from transcript CG9750-RA; CG9750-PA; CG9750-PB; dReptin; rept-PA; rept-PB; reptin

UniProt Comments for rept

Function: Acts as a transcriptional coactivator in Wg signaling caused by altered arm signaling. Pont and rept interfere antagonistically with nuclear arm signaling function, and are required to enhance or reduce arm activity, respectively. Also an essential cofactor for the normal function of Myc; required for cellular proliferation and growth. Ref.1 Ref.5Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair

By similarity. Ref.1 Ref.5

Catalytic activity: ATP + H2O = ADP + phosphate.

Subunit structure: Forms homohexameric rings. May form a dodecamer with rept made of two stacked hexameric rings

By similarity. Component of the chromatin remodeling Ino80 complex. Interacts with dm and pont. Ref.5 Ref.6

Subcellular location: Nucleus Ref.1.

Tissue specificity: Higher expression occurs in primordia of mesoderm, anterior and posterior midgut and cephalic furrow early in gastrulation, as well as in endoderm and mesoderm lineages during germ band extension. Later in development expression is only maintained in endoderm cells. Expressed in thoracic and abdominal segment neural precursors of all embryonic chordotonal organs. Ref.1

Developmental stage: Expressed both maternally and zygotically. Ref.1

Disruption phenotype: Death at first larval instar. Ref.1

Sequence similarities: Belongs to the RuvB family.
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Research Articles on rept

1. The enzymatic functions of Rept and Pont are indispensable for maintaining Hox gene expression states, highlighting the importance of these two antagonistic factors in transcriptional output.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-rept antibody

 Reference Product  PubMed Publications
 pont antibody  >3 publications with rept and pont

Organs/Tissues associated with anti-rept antibody

 Organ/Tissue Name  Pubmed Publications
 Head Antibodies  >1 publications with rept and Head
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