Sheep Anti-SEMA5A Polyclonal Antibody – Cat# MBS629148

Detection of Sema5A: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-h/m/rSema5A.

Semaphorin 5a (SEMA5A) Antibody – Sheep Polyclonal

Name: SEMA5A Antibody; Polyclonal; Sheep; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS629148
Price: 1105 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629148

Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with… More details

$1105 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Semaphorin 5A (SEMA5A), Polyclonal Antibody

Full Product Name

Semaphorin 5A (Semaphorin-5A, Semaphorin F, Sema F, SEMA5A, SEMAF)

Product Gene Name

anti-SEMA5A antibody

Product Synonym Names

Anti -Semaphorin 5A (Semaphorin-5A, Semaphorin F, Sema F, SEMA5A, SEMAF)

Clonality

Polyclonal

Isotype

IgG

Host

Sheep

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human Semaphorin 5A. Species Crossreactivity: mouse and rat.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

120,615 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (9035138..9546233, complement). Location: 5p15.2

OMIM

609297

3D Structure

ModBase 3D Structure for Q13591

Immunogen

Purified, CHO cell-derived, recombinant human Semaphorin 5A (Glu23-Thr765; Accession # AAC09473).

Product Categories/Family for anti-SEMA5A antibody

Antibodies; Abs to Plexin; Semaphorin

Applications Tested/Suitable for anti-SEMA5A antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-SEMA5A antibody

Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: 1ug/ml
ELISA: 0.5-1ug/ml

Other Notes

Small volumes of anti-SEMA5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEMA5A purchase

MBS629148 is an antibody targeting SEMA5A. Also known as Semaphorin 5A (Semaphorin-5A, Semaphorin F, Sema F, SEMA5A, SEMAF). Reactive in Human, Mouse, Rat. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA5A. It may not necessarily be applicable to this product.

NCBI GI #

147904700

NCBI GeneID

9037

NCBI Accession #

NP_003957.2

UniProt Primary Accession #

Q13591

UniProt Secondary Accession #

O60408; Q1RLL9; D3DTC6

UniProt Related Accession #

Q13591

GenBank Nucleotide #

NM_003966.2

NCBI Official Full Name

semaphorin-5A

NCBI Official Synonym Full Names

sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

NCBI Official Symbol

SEMA5A

NCBI Official Synonym Symbols

semF; SEMAF; FLJ12815

UniProt Protein Name

Semaphorin-5A

UniProt Synonym Protein Names

Semaphorin-F

Protein Family

Semaphorin

UniProt Gene Name

SEMA5A

UniProt Synonym Gene Names

SEMAF

UniProt Entry Name

SEM5A_HUMAN

NCBI Protein Information

semaphorin-5A; sema F; semaphorin F; semaphorin-F; OTTHUMP00000115584

NCBI Summary for SEMA5A

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.

UniProt Comments for SEMA5A

SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: syndecan binding; heparan sulfate proteoglycan binding; axon guidance receptor activity; semaphorin receptor binding

Biological Process: nervous system development; axon guidance; positive regulation of actin filament depolymerization; axonal fasciculation; negative regulation of cell adhesion; negative regulation of axon extension involved in axon guidance; diencephalon development; patterning of blood vessels; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive chemotaxis; cell-cell signaling; positive regulation of axon extension involved in axon guidance; positive regulation of endothelial cell proliferation; cell adhesion

Research Articles on SEMA5A

1. The downregulation of SEMA5A in tumor tissue, both at the transcriptional and translational levels, was associated with poor survival among nonsmoking women with NSCLC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SEMA5A antibody

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Other Semaphorin Interactions Pathway antibodies	Other Semaphorin Interactions Pathway Diagram
Semaphorin Interactions Pathway antibodies	Semaphorin Interactions Pathway Diagram

Diseases associated with anti-SEMA5A antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>8 publications with SEMA5A and Neoplasms
Parkinson Disease Antibodies	>5 publications with SEMA5A and Parkinson Disease
Carcinoma Antibodies	>4 publications with SEMA5A and Carcinoma
Stomach Neoplasms Antibodies	>3 publications with SEMA5A and Stomach Neoplasms
Autistic Disorder Antibodies	>2 publications with SEMA5A and Autistic Disorder
Adenocarcinoma Antibodies	>1 publications with SEMA5A and Adenocarcinoma
Inflammation Antibodies	>1 publications with SEMA5A and Inflammation
Lung Neoplasms Antibodies	>1 publications with SEMA5A and Lung Neoplasms

Organs/Tissues associated with anti-SEMA5A antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>27 publications with SEMA5A and Nerve
Brain Antibodies	>13 publications with SEMA5A and Brain
Embryonic Tissue Antibodies	>7 publications with SEMA5A and Embryonic Tissue
Lung Antibodies	>3 publications with SEMA5A and Lung
Stomach Antibodies	>3 publications with SEMA5A and Stomach
Eye Antibodies	>3 publications with SEMA5A and Eye
Bone Antibodies	>2 publications with SEMA5A and Bone
Blood Antibodies	>2 publications with SEMA5A and Blood
Muscle Antibodies	>1 publications with SEMA5A and Muscle
Prostate Antibodies	>1 publications with SEMA5A and Prostate