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AKT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629321

AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15… More details

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Product Overview

Online Datasheet in PDF
Product Name
AKT2, Polyclonal Antibody
Full Product Name
AKT2, ID (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B, beta, PKB beta)
Product Gene Name
Product Synonym Names
Anti -AKT2, ID (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B, beta, PKB beta)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human AKT2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
39,827 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (40736224..40791265, complement). Location: 19q13.1-q13.2
3D Structure
ModBase 3D Structure for Q05BV0
Immunogen
Synthetic peptide selected from the center region of human AKT2 (KLH).
Applications Tested/Suitable for anti-AKT2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-AKT2 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500

Western Blot (WB) of anti-AKT2 antibody

Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
Rabbit Anti-AKT2 Polyclonal Antibody – Cat# MBS629321

Western Blot (WB) of anti-AKT2 antibody

AKT2 Antibody (N108) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AKT2 antibody detected the AKT2 protein (arrow).
Rabbit Anti-AKT2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS629321

Immunofluorescence (IF) of anti-AKT2 antibody

Confocal immunofluorescent analysis of AKT2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-AKT2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS629321

Flow Cytometry (FC/FACS) of anti-AKT2 antibody

AKT2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-AKT2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS629321

Flow Cytometry (FC/FACS) of anti-AKT2 antibody

AKT2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-AKT2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS629321

Other Notes

Small volumes of anti-AKT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKT2 purchase

MBS629321 is an antibody targeting AKT2. Also known as AKT2, ID (RAC-beta Serine/Threonine-protein Kinase, RAC-PK-beta, Protein Kinase Akt-2, Protein Kinase B, beta, PKB beta). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AKT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
AKT2 protein
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol
AKT2
NCBI Official Synonym Symbols
PKBB; PRKBB; PKBBETA; RAC-BETA
UniProt Protein Name
AKT2 protein
Protein Family
UniProt Gene Name
AKT2
UniProt Entry Name
Q05BV0_HUMAN
NCBI Protein Information
RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; OTTHUMP00000174417; protein kinase Akt-2; protein kinase B beta; rac protein kinase beta; murine thymoma viral (v-akt) homolog-2

NCBI Summary for AKT2

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq]
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UniProt Comments for AKT2

Sequence similarities: Belongs to the protein kinase superfamily. RuleBase RU000305Contains 1 PH domain. RuleBase RU003350

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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