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Guanylate Cyclase Alpha-2, Soluble (GUCY1A2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629426

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs. More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Guanylate Cyclase alpha-2, Soluble (GUCY1A2), Polyclonal Antibody
Full Product Name
Guanylate Cyclase alpha-2, Soluble, NT (Guanylate Cyclase Soluble Subunit alpha-2, Guanylate Cyclase 1 Soluble alpha 2, GCS-alpha-2, GC-SA2, GUC1A2, GUCSA2, GUCY1A2)
Product Gene Name
Product Synonym Names
Anti -Guanylate Cyclase alpha-2, Soluble, NT (Guanylate Cyclase Soluble Subunit alpha-2, Guanylate Cyclase 1 Soluble alpha 2, GCS-alpha-2, GC-SA2, GUC1A2, GUCSA2, GUCY1A2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human GUCY1A2. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
81,750 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (106557910..106889171, complement). Location: 11q21-q22
OMIM
601244
3D Structure
ModBase 3D Structure for P33402
Immunogen
Synthetic peptide selected from the N-terminal region of human GUCY1A2 (KLH).
Applications Tested/Suitable for anti-GUCY1A2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GUCY1A2 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:200
Immunohistochemistry: 1:50-1:100

Immunohistochemistry (IHC) of anti-GUCY1A2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-GUCY1A2 Polyclonal Antibody – Cat# MBS629426

Western Blot (WB) of anti-GUCY1A2 antibody

Western blot analysis of anti-GUCY1A2 Antibody (N-term) in mouse cerebellum tissue lysates (35ug/lane). GUCY1A2 (arrow) was detected using the purified Pab.
Rabbit Anti-GUCY1A2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS629426

Other Notes

Small volumes of anti-GUCY1A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GUCY1A2 purchase

MBS629426 is an antibody targeting GUCY1A2. Also known as Guanylate Cyclase alpha-2, Soluble, NT (Guanylate Cyclase Soluble Subunit alpha-2, Guanylate Cyclase 1 Soluble alpha 2, GCS-alpha-2, GC-SA2, GUC1A2, GUCSA2, GUCY1A2). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GUCY1A2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
guanylate cyclase soluble subunit alpha-2
NCBI Official Synonym Full Names
guanylate cyclase 1, soluble, alpha 2
NCBI Official Symbol
GUCY1A2
NCBI Official Synonym Symbols
GC-SA2; GUC1A2
UniProt Protein Name
Guanylate cyclase soluble subunit alpha-2
UniProt Gene Name
GUCY1A2
UniProt Synonym Gene Names
GUC1A2; GUCSA2
UniProt Entry Name
GCYA2_HUMAN
NCBI Protein Information
guanylate cyclase soluble subunit alpha-2; GCS-alpha-2; OTTHUMP00000232532; OTTHUMP00000232533; OTTHUMP00000232534

NCBI Summary for GUCY1A2

Soluble guanylyl (or guanylate) cyclases are heterodimeric enzymes consisting of an alpha subunit, such as alpha-2 (GUCY1A2), and a beta subunit, typically beta-1 (GUCY1B3; MIM 139397), which are activated by nitric oxide (NO) and which catalyze conversion of GTP to 3-prime, 5-prime-cyclic GMP and pyrophosphate (Harteneck et al., 1991 [PubMed 1683630]).[supplied by OMIM]
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UniProt Comments for GUCY1A2

GUCY1A2: Has guanylyl cyclase on binding to the beta-1 subunit. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Nucleotide Metabolism - purine; EC 4.6.1.2; Guanylyl cyclase

Chromosomal Location of Human Ortholog: 11q21-q22

Cellular Component: cytoplasm

Molecular Function: guanylate cyclase activity; GTP binding; heme binding

Biological Process: cGMP biosynthetic process; positive regulation of cGMP biosynthetic process; blood coagulation; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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