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ECSIT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6294283

MBS6294283 is an antibody targeting ECSIT. Also known as ECSIT, CT (ECSIT, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC) (FITC). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ECSIT, Polyclonal Antibody
Full Product Name
ECSIT, CT (ECSIT, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC) (FITC)
Product Gene Name
anti-ECSIT antibody
Product Synonym Names
ECSIT; Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; Protein SITPEC
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
33,938 Da
OMIM
608388
3D Structure
ModBase 3D Structure for Q9BQ95
Immunogen
ECSIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-425 amino acids from the C-terminal region of human ECSIT.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-ECSIT antibody

Antibodies; Adapter Proteins
Applications Tested/Suitable for anti-ECSIT antibody
Western Blot (WB), FLISA
Application Notes for anti-ECSIT antibody
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ECSIT antibody

ECSIT Antibody (C-term) western blot analysis in Jurkat,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ECSIT antibody detected the ECSIT protein (arrow).
Rabbit Anti-ECSIT Polyclonal Antibody – Cat# MBS6294283

Immunofluorescence (IF) of anti-ECSIT antibody

Fluorescent confocal image of Hela cell stained with ECSIT Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ECSIT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ECSIT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Rabbit Anti-ECSIT Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6294283

Product References and Citations for anti-ECSIT antibody

Vogel, R.O., et al. Genes Dev. 21(5):615-624(2007). Xiao, C., et al. Genes Dev. 17(23):2933-2949(2003). Kopp, E., et al. Genes Dev. 13(16):2059-2071(1999).

Other Notes

Small volumes of anti-ECSIT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ECSIT. It may not necessarily be applicable to this product.

NCBI GI #
215599065
NCBI GeneID
51295
NCBI Accession #
NP_001135936.1
NCBI Related Accession #
NP_057665.2
UniProt Primary Accession #
Q9BQ95
UniProt Secondary Accession #
Q96HQ7; Q9NYI1; E9PAN9; K7EMM0
UniProt Related Accession #
Q9BQ95
GenBank Nucleotide #
NM_001142464.2
NCBI Official Full Name
evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial isoform 2
NCBI Official Synonym Full Names
ECSIT signalling integrator
NCBI Official Symbol
ECSIT
NCBI Official Synonym Symbols
SITPEC
UniProt Protein Name
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
UniProt Synonym Protein Names
Protein SITPEC
Protein Family
Evolutionarily conserved signaling intermediate
UniProt Gene Name
ECSIT
UniProt Entry Name
ECSIT_HUMAN
NCBI Protein Information
evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; ECSIT homolog; likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved

UniProt Comments for ECSIT

ECSIT: Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. Belongs to the ECSIT family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; transcription factor complex; mitochondrion; cytoplasm; nucleus

Molecular Function: signal transducer activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; transcription factor activity

Biological Process: BMP signaling pathway; mesoderm formation; innate immune response; regulation of oxidoreductase activity
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Research Articles on ECSIT

1. TRIM59 interacts with ECSIT as an adaptor protein required for the TLR-mediated transduction pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ECSIT antibody

 Reference Product  PubMed Publications
 TRAF6 antibody  >4 publications with ECSIT and TRAF6
 NDUFAF1 antibody  >3 publications with ECSIT and NDUFAF1
 MAP3K1 antibody  >2 publications with ECSIT and MAP3K1
 IRAK2 antibody  >1 publications with ECSIT and IRAK2

Pathways associated with anti-ECSIT antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MyD88 Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88 Cascade Initiated On Plasma Membrane Pathway Diagram
 MyD88 Dependent Cascade Initiated On Endosome Pathway antibodies  MyD88 Dependent Cascade Initiated On Endosome Pathway Diagram
 MyD88:Mal Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88:Mal Cascade Initiated On Plasma Membrane Pathway Diagram
 TRAF6 Mediated Induction Of NFkB And MAP Kinases Upon TLR7/8 Or 9 Activation Pathway antibodies  TRAF6 Mediated Induction Of NFkB And MAP Kinases Upon TLR7/8 Or 9 Activation Pathway Diagram

Diseases associated with anti-ECSIT antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with ECSIT and Nervous System Diseases
 Inflammation Antibodies  >4 publications with ECSIT and Inflammation
 Necrosis Antibodies  >2 publications with ECSIT and Necrosis
 Neurobehavioral Manifestations Antibodies  >1 publications with ECSIT and Neurobehavioral Manifestations
 Heart Diseases Antibodies  >1 publications with ECSIT and Heart Diseases

Organs/Tissues associated with anti-ECSIT antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >5 publications with ECSIT and Bone
 Heart Antibodies  >3 publications with ECSIT and Heart
 Brain Antibodies  >2 publications with ECSIT and Brain
 Vascular Antibodies  >1 publications with ECSIT and Vascular
 Pancreas Antibodies  >1 publications with ECSIT and Pancreas
 Kidney Antibodies  >1 publications with ECSIT and Kidney
 Stomach Antibodies  >1 publications with ECSIT and Stomach
 Liver Antibodies  >1 publications with ECSIT and Liver
 Intestine Antibodies  >1 publications with ECSIT and Intestine
 Lymph Antibodies  >1 publications with ECSIT and Lymph
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