• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

EHD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6294768

MBS6294768 is an antibody targeting EHD3. Also known as EHD3, NT (EHD3, EHD2, PAST3, EH domain-containing protein 3, PAST homolog 3) (HRP). Reactive in Human. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
EHD3, Polyclonal Antibody
Full Product Name
EHD3, NT (EHD3, EHD2, PAST3, EH domain-containing protein 3, PAST homolog 3) (HRP)
Product Gene Name
anti-EHD3 antibody
Product Synonym Names
EHD3; EHD2; PAST3; EH domain-containing protein 3; PAST homolog 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
46,059 Da
OMIM
605890
3D Structure
ModBase 3D Structure for Q9NZN3
Immunogen
EHD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human EHD3.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-EHD3 antibody

Antibodies; Proteins
Applications Tested/Suitable for anti-EHD3 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-EHD3 antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-EHD3 antibody

Western blot analysis of EHD3 Antibody (N-term) in mouse lung tissue lysates (35ug/lane).EHD3 (arrow) was detected using the purified Pab.
Rabbit Anti-EHD3 Polyclonal Antibody – Cat# MBS6294768

Immunohistochemistry (IHC) of anti-EHD3 antibody

EHD3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EHD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-EHD3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6294768

Flow Cytometry (FC/FACS) of anti-EHD3 antibody

EHD3 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-EHD3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6294768

Product References and Citations for anti-EHD3 antibody

Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009). Naslavsky, N., et al. J. Cell. Sci. 122 (PT 3), 389-400 (2009). Roland, J.T., et al. Mol. Biol. Cell 18(8):2828-2837(2007).

Other Notes

Small volumes of anti-EHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EHD3. It may not necessarily be applicable to this product.

NCBI GI #
21361462
NCBI GeneID
30846
NCBI Accession #
NP_055416.2
UniProt Primary Accession #
Q9NZN3
UniProt Secondary Accession #
Q96CB6; B2RDH9; B4DNU6
UniProt Related Accession #
Q9NZN4
GenBank Nucleotide #
NM_014601.3
NCBI Official Full Name
EH domain-containing protein 2
NCBI Official Synonym Full Names
EH-domain containing 2
NCBI Official Symbol
EHD2
NCBI Official Synonym Symbols
PAST2
UniProt Protein Name
EH domain-containing protein 2
UniProt Synonym Protein Names
PAST homolog 2Curated
Protein Family
EH domain-containing protein
UniProt Gene Name
EHD2
UniProt Entry Name
EHD2_HUMAN
NCBI Protein Information
EH domain-containing protein 2; PAST homolog 2; EH domain containing 2

NCBI Summary for EHD3

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for EHD3

EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome membrane

Molecular Function: ATP binding; calcium ion binding; GTP binding; hydrolase activity; nucleic acid binding; protein binding; protein domain specific binding

Biological Process: blood coagulation; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endocytosis; metabolic process
Show more

Research Articles on EHD3

1. Phosphatidylinositol 4,5-bisphosphate controls EHD2 plasma membrane localization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EHD3 antibody

 Reference Product  PubMed Publications
 EHD1 antibody  >23 publications with EHD3 and EHD1
 EHD3 antibody  >12 publications with EHD3 and EHD3
 PACSIN2 antibody  >5 publications with EHD3 and PACSIN2
 EHBP1 antibody  >5 publications with EHD3 and EHBP1
 SLC2A4 antibody  >2 publications with EHD3 and SLC2A4
 CAV1 antibody  >1 publications with EHD3 and CAV1
 FER1L5 antibody  >1 publications with EHD3 and FER1L5

Pathways associated with anti-EHD3 antibody

 Products by Pathway  Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with anti-EHD3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with EHD3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >2 publications with EHD3 and Disease Models, Animal
 Brain Diseases Antibodies  >1 publications with EHD3 and Brain Diseases
 Skin Diseases Antibodies  >1 publications with EHD3 and Skin Diseases
 Breast Neoplasms Antibodies  >1 publications with EHD3 and Breast Neoplasms
 Ovarian Diseases Antibodies  >1 publications with EHD3 and Ovarian Diseases
 Myocardial Infarction Antibodies  >1 publications with EHD3 and Myocardial Infarction
 Ovarian Neoplasms Antibodies  >1 publications with EHD3 and Ovarian Neoplasms

Organs/Tissues associated with anti-EHD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >9 publications with EHD3 and Muscle
 Brain Antibodies  >5 publications with EHD3 and Brain
 Vascular Antibodies  >2 publications with EHD3 and Vascular
 Heart Antibodies  >2 publications with EHD3 and Heart
 Connective Tissue Antibodies  >2 publications with EHD3 and Connective Tissue
 Adipose Tissue Antibodies  >2 publications with EHD3 and Adipose Tissue
 Liver Antibodies  >1 publications with EHD3 and Liver
 Stomach Antibodies  >1 publications with EHD3 and Stomach
 Pancreas Antibodies  >1 publications with EHD3 and Pancreas
 Kidney Antibodies  >1 publications with EHD3 and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000