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AKAP 95 (AKAP3) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629576

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene… More details

$980 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
AKAP 95 (AKAP3), Polyclonal Antibody
Full Product Name
AKAP 95 (A Kinase Protein 8, AKAP8, AKAP-8, A Kinase (PRKA) Anchor Protein 8, AKAP95, A Kinase (PRKA) Anchor Protein 95kD)
Product Gene Name
Product Synonym Names
Anti -AKAP 95 (A Kinase Protein 8, AKAP8, AKAP-8, A Kinase (PRKA) Anchor Protein 8, AKAP95, A Kinase (PRKA) Anchor Protein 95kD)
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human AKAP95 at ~100kD in Human Liver lysate after 1ug/ml antibody staining. This band corresponds to the 95kD band reported in literature (PMID
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
94,751 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (4723837..4758213, complement). Location: 12p13.3
OMIM
604689
3D Structure
ModBase 3D Structure for O75969
Immunogen
Synthetic peptide corresponding to AKAP95.
Applications Tested/Suitable for anti-AKAP3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-AKAP3 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32000
Western Blot: 1-3ug/ml

Western Blot (WB) of anti-AKAP3 antibody

Western blot analysis of AKAP95 in Human Liver lysate (RIPA buffer, 35μg total protein per lane) using AKAP95 antibody (1μg/ml).
Goat Anti-AKAP3 Polyclonal Antibody – Cat# MBS629576

Other Notes

Small volumes of anti-AKAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKAP3 purchase

MBS629576 is an antibody targeting AKAP3. Also known as AKAP 95 (A Kinase Protein 8, AKAP8, AKAP-8, A Kinase (PRKA) Anchor Protein 8, AKAP95, A Kinase (PRKA) Anchor Protein 95kD). Reactive in Human, Mouse, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for AKAP3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
A-kinase anchor protein 3
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 3
NCBI Official Symbol
AKAP3
NCBI Official Synonym Symbols
CT82; SOB1; FSP95; PRKA3; AKAP110; AKAP 110
UniProt Protein Name
A-kinase anchor protein 3
UniProt Synonym Protein Names
A-kinase anchor protein 110 kDa; AKAP 110; Cancer/testis antigen 82; CT82; Fibrous sheath protein of 95 kDa; FSP95; Fibrousheathin I; Fibrousheathin-1; Protein kinase A-anchoring protein 3; PRKA3; Sperm oocyte-binding protein
UniProt Gene Name
AKAP3
UniProt Synonym Gene Names
AKAP110; SOB1; AKAP-3; AKAP 110; CT82; FSP95; PRKA3
UniProt Entry Name
AKAP3_HUMAN
NCBI Protein Information
A-kinase anchor protein 3; fibrousheathin I; fibrousheathin-1; cancer/testis antigen 82; sperm oocyte-binding protein 1; A-kinase anchor protein, 110kDa; Fibrous Sheath Protein of 95 kDa; protein kinase A-anchoring protein 3; protein kinase A binding protein AKAP 110

NCBI Summary for AKAP3

This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction. [provided by RefSeq, May 2013]
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UniProt Comments for AKAP3

AKAP3: May function as a regulator of both motility- and head- associated functions such as capacitation and the acrosome reaction. Belongs to the AKAP110 family.

Protein type: Adaptor/scaffold; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: cytoplasm; acrosome; nucleus

Molecular Function: protein binding; protein kinase A binding

Biological Process: protein localization; single fertilization; acrosome reaction; transmembrane receptor protein serine/threonine kinase signaling pathway; cell motility
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AKAP3 antibody

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