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Heat Shock Protein 27 (HSP27) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629671

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels… More details

$545 / 0.02 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Heat Shock Protein 27 (Hsp27), Polyclonal Antibody
Full Product Name
Heat Shock Protein 27, phosphorylated (Ser82) (HSP27)
Product Gene Name
Product Synonym Names
Anti -Heat Shock Protein 27, phosphorylated (Ser82) (HSP27)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Specificity
Detects endogenous HSP27 only when phosphorylated at Ser82. The antibody does not recognize other heat shock proteins.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: 3L; NT_037436.3 (9377165..9378384). Location: 67B3-67B3
Immunogen
Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser82 of human HSP27.
Applications Tested/Suitable for anti-Hsp27 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Hsp27 antibody
Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry.
Dilution: Flow Cytometry: 1:25
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Immunohistochemistry (Frozen): 1:50
Immunofluorescence: 1:25

Western Blot (WB) of anti-Hsp27 antibody

Western blot analysis of extracts from HeLa and PC12 cells, treated with λ-phosphatase or UV as indicated, using MBS629671
Rabbit Anti-HSP27 Polyclonal Antibody – Cat# MBS629671

Immunohistochemistry (IHC) of anti-Hsp27 antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MBS629671
Rabbit Anti-HSP27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629671

Immunohistochemistry (IHC) of anti-Hsp27 antibody

Immunohistochemical analysis of paraffin-embedded human melanoma using MBS629671
Rabbit Anti-HSP27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629671

Immunohistochemistry (IHC) of anti-Hsp27 antibody

Immunohistochemical analysis of frozen H1650 xenograft using MBS629671
Rabbit Anti-HSP27 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629671

Flow Cytometry (FC/FACS) of anti-Hsp27 antibody

Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using MBS629671
Rabbit Anti-HSP27 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS629671

Immunofluorescence (IF) of anti-Hsp27 antibody

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using MBS629671 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Rabbit Anti-HSP27 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS629671

Other Notes

Small volumes of anti-Hsp27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hsp27 purchase

MBS629671 is an antibody targeting Hsp27. Also known as Heat Shock Protein 27, phosphorylated (Ser82) (HSP27). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
heat shock protein 27
NCBI Official Synonym Full Names
Heat shock protein 27
NCBI Official Symbol
Hsp27
NCBI Official Synonym Symbols
27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
Protein Family
NCBI Protein Information
CG4466 gene product from transcript CG4466-RA; heat shock protein 27; CG4466-PA; Hsp27-PA; heat shock protein hsp27; Hsp 27

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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