Rabbit Anti-eNOS Polyclonal Antibody – Cat# MBS629859

IHC staining of paraffin-embedded human placenta using MBS629859 (right) compared to a negative control without primary antibody(left). HIER was performed using sodium citrate, pH 6.0. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol, washed and then probed with the anibody at 1:20 dilution, with overnight incubation at 4°C. HRP-conjugated secondary antibody with DAB detection was used.

Phosphorylated (eNOS) Antibody – Rabbit Polyclonal

Name: eNOS Antibody; Polyclonal; Rabbit; Bovine-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS629859
Price: 910 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629859

The oxidation of L-arginine by a family of nitric oxide synthase (NOS) isoenzymes, nNOS, eNOS, and iNOS, is responsible for the production of nitric oxide (NO). In mammalian genomes, three separate genes encode the NOS isoforms, two expressed constitutively… More details

$910 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

eNOS Phosphorylated, Polyclonal Antibody

Full Product Name

eNOS Phosphorylated (Ser1179) (NOS III, Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothe

Product Gene Name

anti-eNOS antibody

Product Synonym Names

Anti -eNOS Phosphorylated (Ser1179) (NOS III, Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothe

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Bovine

Form/Format

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes bovine eNOS protein, phosphorylated at Ser1179 (~135kD). Reactivity has been con rmed with VEGF-stimulated, serum-starved BAEC (bovine aortic endothelial cell) lysates. A very low signal was observed in the lysates of serum-starved, unstimulated BAEC and no signal was observed in lysates of unstimulated BAEC in the presence of serum. Species Sequence Homology: human, rat, mouse, canine, porcine and guinea pig

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

5,517 Da

3D Structure

ModBase 3D Structure for A6H544

Immunogen

Synthetic peptide corresponding to the C-terminal region of the bovine eNOS (endothelial nitric oxide synthase, NOS III) protein, phosphorylated at Ser1179.

Product Categories/Family for anti-eNOS antibody

Antibodies; Abs to Nitric Oxide

Applications Tested/Suitable for anti-eNOS antibody

ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)

Application Notes for anti-eNOS antibody

Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immuno uorescence: Reactive with monkey COS cells (1)

Other Notes

Small volumes of anti-eNOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eNOS purchase

MBS629859 is an antibody targeting eNOS. Also known as eNOS Phosphorylated (Ser1179) (NOS III, Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothe. Reactive in Bovine. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for eNOS. It may not necessarily be applicable to this product.

NCBI GI #

148913721

UniProt Primary Accession #

A6H544

NCBI Official Full Name

eNOS protein

UniProt Protein Name

ENOS protein

UniProt Gene Name

eNOS

UniProt Entry Name

A6H544_CALJA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-eNOS antibody

Reference Product	PubMed Publications
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