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Calponin-3 (CNN3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629871

Calponin-3 is a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. This protein is associated with the cytoskeleton but is not involved in contraction. More details

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Product Overview

Online Datasheet in PDF
Product Name
Calponin-3 (CNN3), Polyclonal Antibody
Full Product Name
Calponin-3, NT (Calponin 3, Calponin, Acidic Isoform, CNN3)
Product Gene Name
Product Synonym Names
Anti -Calponin-3, NT (Calponin 3, Calponin, Acidic Isoform, CNN3)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human Calponin 3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
36,414 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (95362507..95392735, complement). Location: 1p22-p21
OMIM
602374
3D Structure
ModBase 3D Structure for Q15417
Immunogen
Synthetic peptide selected from the N-terminal region of human Calponin 3 (KLH).
Applications Tested/Suitable for anti-CNN3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-CNN3 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-CNN3 antibody

Western blot analysis of Calponin-3 Antibody (N-term) in A2058 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.
Rabbit Anti-CNN3 Polyclonal Antibody – Cat# MBS629871

Immunohistochemistry (IHC) of anti-CNN3 antibody

Calponin-3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CNN3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629871

Flow Cytometry (FC/FACS) of anti-CNN3 antibody

Calponin-3 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CNN3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS629871

Other Notes

Small volumes of anti-CNN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CNN3 purchase

MBS629871 is an antibody targeting CNN3. Also known as Calponin-3, NT (Calponin 3, Calponin, Acidic Isoform, CNN3). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CNN3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calponin-3
NCBI Official Synonym Full Names
calponin 3, acidic
NCBI Official Symbol
CNN3
UniProt Protein Name
Calponin-3
UniProt Synonym Protein Names
Calponin, acidic isoform
Protein Family
UniProt Gene Name
CNN3
UniProt Entry Name
CNN3_HUMAN
NCBI Protein Information
calponin-3; OTTHUMP00000012470; OTTHUMP00000012471; calponin, acidic isoform; dJ639P13.2.2 (acidic calponin 3)

NCBI Summary for CNN3

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq]
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UniProt Comments for CNN3

calponin 3: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton

Molecular Function: calmodulin binding; actin binding

Biological Process: epithelial cell differentiation; actomyosin structure organization and biogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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