• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

TIMP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629888

TIMP1 belongs to the TIMP family. The proteins in this family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TIMP1, Polyclonal Antibody
Full Product Name
TIMP1, NT (Metalloproteinase Inhibitor 1, Tissue Inhibitor Of Metalloproteinases 1, TIMP-1, Erythroid-potentiating Activity, EPA, Fibroblast Collagenase Inhibitor, Collagenase Inhibitor, TIMP, CLGI)
Product Gene Name
anti-TIMP1 antibody
Product Synonym Names
Anti -TIMP1, NT (Metalloproteinase Inhibitor 1, Tissue Inhibitor Of Metalloproteinases 1, TIMP-1, Erythroid-potentiating Activity, EPA, Fibroblast Collagenase Inhibitor, Collagenase Inhibitor, TIMP, CLGI)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human TIMP1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
23,171 Da
Chromosome Location
Chromosome: X; NC_000023.10 (47441690..47446190). Location: Xp11.3-p11.23
3D Structure
ModBase 3D Structure for Q6FGX5
Immunogen
Synthetic peptide corresponding to a region between aa50-80 from the N-terminal region of human TIMP1 conjugated to KLH. Cellular Localization: secreted
Cellular Localization
Secreted

Product Categories/Family for anti-TIMP1 antibody

Antibodies; Abs to Matrix Metalloproteinases
Applications Tested/Suitable for anti-TIMP1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-TIMP1 antibody
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry.
Dilution: Western Blot: 1:50-1:100
ELISA: 1:1000
Flow Cytometry: 1:10-1:50
Immunofluorescence: 1:10-1:50

Western Blot (WB) of anti-TIMP1 antibody

Western blot analysis of TIMP1 Antibody (N-term) in CEM cell line lysates (35ug/lane). TIMP1 (arrow) was detected using the purified Pab.
Rabbit Anti-TIMP1 Polyclonal Antibody – Cat# MBS629888

Immunohistochemistry (IHC) of anti-TIMP1 antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-TIMP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629888

Flow Cytometry (FC/FACS) of anti-TIMP1 antibody

Flow cytometric analysis of MDA-231 cells using TIMP1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-TIMP1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS629888

Immunofluorescence (IF) of anti-TIMP1 antibody

Confocal immunofluorescent analysis of TIMP1 Antibody (N-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-TIMP1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS629888

Other Notes

Small volumes of anti-TIMP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIMP1 purchase

MBS629888 is an antibody targeting TIMP1. Also known as TIMP1, NT (Metalloproteinase Inhibitor 1, Tissue Inhibitor Of Metalloproteinases 1, TIMP-1, Erythroid-potentiating Activity, EPA, Fibroblast Collagenase Inhibitor, Collagenase Inhibitor, TIMP, CLGI). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TIMP1. It may not necessarily be applicable to this product.

NCBI GI #
49456917
NCBI GeneID
7076
UniProt Primary Accession #
Q6FGX5
UniProt Related Accession #
P01033; Q58P21; Q5H9A7; Q5H9A8; Q6FGX5
NCBI Official Full Name
TIMP1
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1
NCBI Official Symbol
TIMP1
NCBI Official Synonym Symbols
EPA; EPO; HCI; CLGI; TIMP; FLJ90373
UniProt Protein Name
TIMP metallopeptidase inhibitor 1
Protein Family
Metalloproteinase inhibitor
UniProt Gene Name
TIMP1
UniProt Entry Name
Q6FGX5_HUMAN
NCBI Protein Information
metalloproteinase inhibitor 1; TIMP-1; OTTHUMP00000023214; OTTHUMP00000023216; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1

NCBI Summary for TIMP1

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq]
Show more

UniProt Comments for TIMP1

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: Xp11.3-p11.23

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; growth factor activity; metal ion binding; cytokine activity

Biological Process: platelet activation; response to peptide hormone stimulus; extracellular matrix disassembly; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; blood coagulation; negative regulation of apoptosis; aging
Show more

Research Articles on TIMP1

1. TIMP-1 expression had no relationship with lymph node metastasis in nasopharyngeal carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TIMP1 antibody

 Reference Product  PubMed Publications
 MMP2 antibody  >125 publications with TIMP1 and MMP2
 MMP1 antibody  >66 publications with TIMP1 and MMP1
 MMP3 antibody  >41 publications with TIMP1 and MMP3

Pathways associated with anti-TIMP1 antibody

 Products by Pathway  Pathway Diagram
 Formation Of Platelet Plug Pathway antibodies  Formation Of Platelet Plug Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL6-mediated Signaling Events Pathway antibodies  IL6-mediated Signaling Events Pathway Diagram
 Matrix Metalloproteinases Pathway antibodies  Matrix Metalloproteinases Pathway Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram

Diseases associated with anti-TIMP1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >108 publications with TIMP1 and Neoplasms
 Fibrosis Antibodies  >52 publications with TIMP1 and Fibrosis
 Carcinoma Antibodies  >48 publications with TIMP1 and Carcinoma
 Cardiovascular Diseases Antibodies  >48 publications with TIMP1 and Cardiovascular Diseases
 Inflammation Antibodies  >38 publications with TIMP1 and Inflammation
 Lung Diseases Antibodies  >32 publications with TIMP1 and Lung Diseases
 Neoplasm Metastasis Antibodies  >29 publications with TIMP1 and Neoplasm Metastasis
 Neoplasm Invasiveness Antibodies  >27 publications with TIMP1 and Neoplasm Invasiveness
 Liver Diseases Antibodies  >26 publications with TIMP1 and Liver Diseases
 Female Urogenital Diseases Antibodies  >22 publications with TIMP1 and Female Urogenital Diseases

Organs/Tissues associated with anti-TIMP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >76 publications with TIMP1 and Blood
 Lung Antibodies  >47 publications with TIMP1 and Lung
 Vascular Antibodies  >46 publications with TIMP1 and Vascular
 Bone Antibodies  >42 publications with TIMP1 and Bone
 Muscle Antibodies  >41 publications with TIMP1 and Muscle
 Liver Antibodies  >41 publications with TIMP1 and Liver
 Heart Antibodies  >31 publications with TIMP1 and Heart
 Embryonic Tissue Antibodies  >27 publications with TIMP1 and Embryonic Tissue
 Connective Tissue Antibodies  >25 publications with TIMP1 and Connective Tissue
 Kidney Antibodies  >24 publications with TIMP1 and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000