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C21ORF33 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630113

HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. HES1 has a particular type of basic domain that contains a helix interrupting protein… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HES1 (C21orf33), Polyclonal Antibody
Full Product Name
HES1, NT (Hairy and Enhancer of Split 1, HES-1, bHLHb39, Class B Basic Helix-loop-helix Protein 39, FLJ20408, Hairy Homolog, Hairy-like Protein, hHL, HL, HRY, Transcription Factor HES-1)
Product Gene Name
Product Synonym Names
Anti -HES1, NT (Hairy and Enhancer of Split 1, HES-1, bHLHb39, Class B Basic Helix-loop-helix Protein 39, FLJ20408, Hairy Homolog, Hairy-like Protein, hHL, HL, HRY, Transcription Factor HES-1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide
Purity/Purification
Purified
Purified by ammonium sulfate precipitation
Specificity
Recognizes human HES1
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
28,170 Da
OMIM
601659
3D Structure
ModBase 3D Structure for P30042
Immunogen
KLH-conjugated synthetic peptide derived from a portion of amino acids corresponding to aa16-46 in the N-terminal region of human HES1
Applications Tested/Suitable for anti-C21orf33 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-C21orf33 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-C21orf33 antibody

Western blot analysis of anti-HES1 Antibody (N-term T24) (RB13980) in HL60 cell line lysates (35ug/lane). HES1 (arrow) was detected using the purified Pab.
Rabbit Anti-C21ORF33 Polyclonal Antibody – Cat# MBS630113

Immunofluorescence (IF) of anti-C21orf33 antibody

Confocal immunofluorescent analysis of HES1 Antibody (N-term T24) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-C21ORF33 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS630113

Flow Cytometry (FC/FACS) of anti-C21orf33 antibody

HES1 Antibody (N-term T24) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-C21ORF33 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS630113

Other Notes

Small volumes of anti-C21orf33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C21orf33 purchase

MBS630113 is an antibody targeting C21orf33. Also known as HES1, NT (Hairy and Enhancer of Split 1, HES-1, bHLHb39, Class B Basic Helix-loop-helix Protein 39, FLJ20408, Hairy Homolog, Hairy-like Protein, hHL, HL, HRY, Transcription Factor HES-1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for C21orf33. It may not necessarily be applicable to this product.

NCBI GI #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
HES1
UniProt Protein Name
ES1 protein homolog, mitochondrial
UniProt Synonym Protein Names
Protein GT335; Protein KNP-I
Protein Family
UniProt Gene Name
C21orf33
UniProt Synonym Gene Names
HES1; KNPI
UniProt Entry Name
ES1_HUMAN

NCBI Summary for C21orf33

This gene encodes a potential mitochondrial protein that is a member of the DJ-1/PfpI gene family. This protein is overexpressed in fetal Down syndrome brain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

UniProt Comments for C21orf33

C21orf33: a mitochondrial protein. Two alternatively spliced isoforms have been described.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: mitochondrion

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-C21orf33 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with C21orf33 and FLNA
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