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GPAA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6303420

MBS6303420 is an antibody targeting GPAA1. Also known as GPAA1, NT (GPAA1, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1 protein homolog) (MaxLight 490). Reactive in Human. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPAA1, Polyclonal Antibody
Full Product Name
GPAA1, NT (GPAA1, GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1 protein homolog) (MaxLight 490)
Product Gene Name
anti-GPAA1 antibody
Product Synonym Names
GPAA1; GAA1; Glycosylphosphatidylinositol anchor attachment 1 protein; GAA1 protein homolog
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human GPAA1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
67,623 Da
OMIM
603048
3D Structure
ModBase 3D Structure for O43292
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 46-73 in the N-terminal region of human GPAA1, Swiss/UniProt Accession: O43292.
Conjugate
MaxLight490
Note
Preservative Free

Product Categories/Family for anti-GPAA1 antibody

Antibodies; Proteins
Applications Tested/Suitable for anti-GPAA1 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-GPAA1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-GPAA1 antibody

Western Blot analysis in U251 cell line lysates (35ug/lane) usingMBS6009573. This demonstrates that MBS6009573 detected the GPAA1 protein (arrow).
Rabbit Anti-GPAA1 Polyclonal Antibody – Cat# MBS6303420

Immunohistochemistry (IHC) of anti-GPAA1 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle using MBS6009573 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6009573 for immunohistochemistry.
Rabbit Anti-GPAA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6303420

Flow Cytometry (FC/FACS) of anti-GPAA1 antibody

Flow Cytometric analysis of U251 cells using MBS6009573 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-GPAA1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6303420

Product References and Citations for anti-GPAA1 antibody

1. Jiang, W.W., et al. Mol. Cancer 6, 74 (2007). 2. Olsen, J.V., et al. Cell 127(3):635-648(2006). 3. Ho, J.C., et al. Int. J. Cancer 119(6):1330-1337(2006). 4. Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005). 5. Vainauskas, S., et al. J. Biol. Chem. 279(8):6540-6545(2004).

Other Notes

Small volumes of anti-GPAA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPAA1. It may not necessarily be applicable to this product.

NCBI GI #
4504079
NCBI GeneID
8733
NCBI Accession #
NP_003792.1
UniProt Primary Accession #
O43292
UniProt Secondary Accession #
Q9NSS0; Q9UQ31
UniProt Related Accession #
O43292
GenBank Nucleotide #
NM_003801.3
NCBI Official Full Name
glycosylphosphatidylinositol anchor attachment 1 protein
NCBI Official Synonym Full Names
glycosylphosphatidylinositol anchor attachment 1
NCBI Official Symbol
GPAA1
NCBI Official Synonym Symbols
GAA1; hGAA1
UniProt Protein Name
Glycosylphosphatidylinositol anchor attachment 1 protein
UniProt Synonym Protein Names
GAA1 protein homolog
Protein Family
Glycosylphosphatidylinositol anchor attachment 1 protein
UniProt Gene Name
GPAA1
UniProt Synonym Gene Names
GAA1; GPI anchor attachment protein 1; hGAA1
UniProt Entry Name
GPAA1_HUMAN
NCBI Protein Information
glycosylphosphatidylinositol anchor attachment 1 protein; GAA1 protein homolog; GPI transamidase subunit; GPI anchor attachment protein 1; GPAA1P anchor attachment protein 1 homolog; glycophosphatidylinositol anchor attachment 1; anchor attachment protein

NCBI Summary for GPAA1

Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008]
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UniProt Comments for GPAA1

GPAA1: Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Endoplasmic reticulum; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: endoplasmic reticulum membrane; membrane; GPI-anchor transamidase complex

Molecular Function: tubulin binding; protein binding; GPI-anchor transamidase activity

Biological Process: cellular protein metabolic process; attachment of GPI anchor to protein; protein complex assembly; C-terminal protein lipidation; post-translational protein modification; protein retention in ER
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Research Articles on GPAA1

1. Results show an increased expression level and elevated copy number for GAA1 in head and neck squamous carcinoma, suggesting a role for this GPI anchor subunit in HNSCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GPAA1 antibody

 Reference Product  PubMed Publications
 PIGK antibody  >5 publications with GPAA1 and PIGK
 PIGO antibody  >1 publications with GPAA1 and PIGO
 PIGT antibody  >1 publications with GPAA1 and PIGT

Pathways associated with anti-GPAA1 antibody

 Products by Pathway  Pathway Diagram
 Attachment Of GPI Anchor To UPAR Pathway antibodies  Attachment Of GPI Anchor To UPAR Pathway Diagram
 Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies  Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram
 Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies  Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway antibodies  Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram

Diseases associated with anti-GPAA1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >6 publications with GPAA1 and Neoplasms
 Nervous System Diseases Antibodies  >5 publications with GPAA1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >4 publications with GPAA1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >2 publications with GPAA1 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >2 publications with GPAA1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >2 publications with GPAA1 and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with GPAA1 and Liver Diseases
 Heart Diseases Antibodies  >2 publications with GPAA1 and Heart Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with GPAA1 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >1 publications with GPAA1 and Inflammation

Organs/Tissues associated with anti-GPAA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3 publications with GPAA1 and Liver
 Brain Antibodies  >3 publications with GPAA1 and Brain
 Bladder Antibodies  >2 publications with GPAA1 and Bladder
 Bone Antibodies  >1 publications with GPAA1 and Bone
 Muscle Antibodies  >1 publications with GPAA1 and Muscle
 Heart Antibodies  >1 publications with GPAA1 and Heart
 Embryonic Tissue Antibodies  >1 publications with GPAA1 and Embryonic Tissue
 Uterus Antibodies  >1 publications with GPAA1 and Uterus
 Ovary Antibodies  >1 publications with GPAA1 and Ovary
 Lung Antibodies  >1 publications with GPAA1 and Lung
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