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PIGK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630466

GPI8 is a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a… More details

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Product Overview

Online Datasheet in PDF
Product Name
GPI8 (PIGK), Polyclonal Antibody
Full Product Name
GPI8, CT (GPI-anchor Transamidase, GPI Transamidase, Phosphatidylinositol-glycan Biosynthesis, Class K Protein, PIG-K, hGPI8, GPI8 Homolog, PIGK)
Product Gene Name
anti-PIGK antibody
Product Synonym Names
Anti -GPI8, CT (GPI-anchor Transamidase, GPI Transamidase, Phosphatidylinositol-glycan Biosynthesis, Class K Protein, PIG-K, hGPI8, GPI8 Homolog, PIGK)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human GPI8. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
45,252 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (77554666..77685132, complement). Location: 1p31.1
OMIM
605087
3D Structure
ModBase 3D Structure for Q92643
Immunogen
Synthetic peptide selected from the C-terminal region of human GPI8 (KLH).

Product Categories/Family for anti-PIGK antibody

Antibodies; Abs to Enzymes
Applications Tested/Suitable for anti-PIGK antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PIGK antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-PIGK antibody

The anti-GPI8 Pab is used in Western blot to detect GPI8 in mouse liver tissue lysate.
Rabbit Anti-PIGK Polyclonal Antibody – Cat# MBS630466

Immunohistochemistry (IHC) of anti-PIGK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-PIGK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630466

Other Notes

Small volumes of anti-PIGK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIGK purchase

MBS630466 is an antibody targeting PIGK. Also known as GPI8, CT (GPI-anchor Transamidase, GPI Transamidase, Phosphatidylinositol-glycan Biosynthesis, Class K Protein, PIG-K, hGPI8, GPI8 Homolog, PIGK). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PIGK. It may not necessarily be applicable to this product.

NCBI GI #
23199983
NCBI GeneID
10026
NCBI Accession #
NP_005473.1
UniProt Primary Accession #
Q92643
UniProt Secondary Accession #
O14822; Q5TG77; B2R7K3
UniProt Related Accession #
Q92643
GenBank Nucleotide #
NM_005482.2
NCBI Official Full Name
GPI-anchor transamidase
NCBI Official Synonym Full Names
phosphatidylinositol glycan anchor biosynthesis, class K
NCBI Official Symbol
PIGK
NCBI Official Synonym Symbols
GPI8
UniProt Protein Name
GPI-anchor transamidase
UniProt Synonym Protein Names
GPI8 homolog; hGPI8; Phosphatidylinositol-glycan biosynthesis class K protein
UniProt Gene Name
PIGK
UniProt Synonym Gene Names
GPI8; GPI transamidase; hGPI8; PIG-K
UniProt Entry Name
GPI8_HUMAN
NCBI Protein Information
GPI-anchor transamidase; PIG-K; GPI8 homolog; GPI transamidase subunit; phosphatidylinositol glycan, class K; phosphatidylinositol-glycan biosynthesis class K protein

NCBI Summary for PIGK

This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]
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UniProt Comments for PIGK

PIGK: Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein. Belongs to the peptidase C13 family.

Protein type: Membrane protein, integral; Protease; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: endoplasmic reticulum membrane; membrane; integral to endoplasmic reticulum membrane; GPI-anchor transamidase complex

Molecular Function: protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; GPI-anchor transamidase activity

Biological Process: cellular protein metabolic process; protein folding; attachment of GPI anchor to protein; C-terminal protein lipidation; post-translational protein modification; proteolysis
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Research Articles on PIGK

1. GPI8 and PIG-T form a functionally important intermolecular disulfide bridge

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PIGK antibody

 Reference Product  PubMed Publications
 GPAA1 antibody  >5 publications with PIGK and GPAA1
 PIGF antibody  >1 publications with PIGK and PIGF
 PIGO antibody  >1 publications with PIGK and PIGO
 PIGS antibody  >1 publications with PIGK and PIGS

Pathways associated with anti-PIGK antibody

 Products by Pathway  Pathway Diagram
 Attachment Of GPI Anchor To UPAR Pathway antibodies  Attachment Of GPI Anchor To UPAR Pathway Diagram
 Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies  Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram
 Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies  Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway antibodies  Post-translational Modification: Synthesis Of GPI-anchored Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram

Diseases associated with anti-PIGK antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >1 publications with PIGK and Death
 Anemia Antibodies  >1 publications with PIGK and Anemia
 Inflammation Antibodies  >1 publications with PIGK and Inflammation

Organs/Tissues associated with anti-PIGK antibody

 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >3 publications with PIGK and Placenta
 Blood Antibodies  >2 publications with PIGK and Blood
 Lymph Node Antibodies  >1 publications with PIGK and Lymph Node
 Thymus Antibodies  >1 publications with PIGK and Thymus
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