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AMOTL2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630554

AMOTL2 is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
AMOTL2, Polyclonal Antibody
Full Product Name
AMOTL2, ID (Leman Coiled-coil Protein, LCCP, Angiomotin-like Protein 2, KIAA0989)
Product Gene Name
anti-AMOTL2 antibody
Product Synonym Names
Anti -AMOTL2, ID (Leman Coiled-coil Protein, LCCP, Angiomotin-like Protein 2, KIAA0989)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide
Purity/Purification
Purified by Protein A and peptide affinity chromatography
Concentration
0.53 mg/ml (lot specific)
Specificity
Recognizes human AMOTL2
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
11,704 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (134074190..134093406, complement). Location: 3q21-q22
3D Structure
ModBase 3D Structure for Q9Y2J4
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa126-152 in the central region of human AMOTL2. Species Sequence Homology: bovine, rat and mouse

Product Categories/Family for anti-AMOTL2 antibody

Antibodies; Abs to Binding Proteins
Applications Tested/Suitable for anti-AMOTL2 antibody
Western Blot (WB)
Application Notes for anti-AMOTL2 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-AMOTL2 antibody

Western blot analysis of AMOTL2 Antibody (Center) in A375 cell line lysates (35ug/lane). AMOTL2 (arrow) was detected using the purified Pab.
Rabbit Anti-AMOTL2 Polyclonal Antibody – Cat# MBS630554

Western Blot (WB) of anti-AMOTL2 antibody

Western blot analysis of AMOTL2 Antibody (Center) in mouse liver tissue lysates (35ug/lane). AMOTL2 (arrow) was detected using the purified Pab.
Rabbit Anti-AMOTL2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630554

Product References and Citations for anti-AMOTL2 antibody

1. Bratt,A.,et.al., Gene 298(1), 69-77 (2002).

Other Notes

Small volumes of anti-AMOTL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AMOTL2 purchase

Anti-angiogenic research is a leading cancer therapeutic area. AMOTL 2, similar to the related angiomotin, is a protein known to mediate angiostatin signaling pathway. As a circulating product, quantitation, analysis of mechanism and bioavailablity require quality anti AMOTL2 antibodies for western blot, ELISA or IHC reactions. This rabbit anti-AMOTL2 polyclonal species pan-specific IgG makes custom protocols easy and flexible, since either goat anti rabbit or donkey anti rabbit secondary antibodies are readily available products and HRP or ALEXA 174 fluor can be used for revelation of signal. Originally purified from rabbits immunized with species human angiomotin protein 2, the rabbit IgG fractions were further affinity purified as described in the included datasheet and antibody services guide. Excellent shelf life and high titre for WB make this primary antibody an ideal choice for cell lysates or blood analysis for humans or mouse over other commercially available primary antibodies or antibody kits.

Additional Details

NCBI/Uniprot data below describe general gene information for AMOTL2. It may not necessarily be applicable to this product.

NCBI GI #
33872302
NCBI GeneID
51421
NCBI Accession #
NP_057285.3
UniProt Primary Accession #
Q9Y2J4
UniProt Related Accession #
Q8TBR8; Q9Y2J4
NCBI Official Full Name
AMOTL2 protein
NCBI Official Synonym Full Names
angiomotin like 2
NCBI Official Symbol
AMOTL2
NCBI Official Synonym Symbols
LCCP
UniProt Protein Name
AMOTL2 protein
Protein Family
Angiomotin-like protein
UniProt Entry Name
Q8TBR8_HUMAN
NCBI Protein Information
angiomotin-like protein 2; OTTHUMP00000216465; OTTHUMP00000216466; OTTHUMP00000216530; OTTHUMP00000216978; Leman coiled-coil protein

NCBI Summary for AMOTL2

Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motins protein family. [provided by RefSeq]
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UniProt Comments for AMOTL2

AMOTL2: Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC. Belongs to the angiomotin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 3q21-q22

Cellular Component: recycling endosome; tight junction; apical plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: identical protein binding; protein binding

Biological Process: Wnt receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AMOTL2 antibody

 Products by Pathway  Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Hippo Pathway antibodies  Signaling By Hippo Pathway Diagram

Diseases associated with anti-AMOTL2 antibody

 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >1 publications with AMOTL2 and Neovascularization, Pathologic
 Breast Neoplasms Antibodies  >1 publications with AMOTL2 and Breast Neoplasms

Organs/Tissues associated with anti-AMOTL2 antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >2 publications with AMOTL2 and Vascular
 Kidney Antibodies  >2 publications with AMOTL2 and Kidney
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