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Actin, Gamma (ACTG1) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630563

Actin is a 43kD highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the… More details

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Product Overview

Online Datasheet in PDF
Product Name
Actin, gamma (ACTG1), Polyclonal Antibody
Full Product Name
Actin, gamma
Product Gene Name
Product Synonym Names
Anti -Actin, gamma
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid.
Purity/Purification
Serum
Serum
Specificity
Recognizes human gamma actin. Species Crossreactivity: rat and mouse. Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
41,836 Da
Chromosome Location
Chromosome: 10; NC_006097.3 (2014377..2015913). Location: chromosome: 10
3D Structure
ModBase 3D Structure for P53478
Immunogen
Synthetic peptide corresponding EEEIAALVIDNGSGGSG at the N-terminus of gamma actin, conjugated to KLH. Cellular Localization: Cytoplasm and cytoskeleton
Applications Tested/Suitable for anti-ACTG1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-ACTG1 antibody
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1:500-1:2000. Non-specific bands can occur when A0760-45 is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kD band
Immunocytochemistry: 1:500-1:2000. Cultured cells fixed with PFA, then permeabilized with methanol.
Immunohistochemistry: 1:500-1:2000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used

Other Notes

Small volumes of anti-ACTG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTG1 purchase

MBS630563 is an antibody targeting ACTG1. Also known as Actin, gamma. Reactive in Human, Mouse, Rat. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTG1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
actin, cytoplasmic type 5
NCBI Official Synonym Full Names
Actin, gamma 1
NCBI Official Symbol
ACTG1
NCBI Official Synonym Symbols
actin; cytoplasmic
UniProt Protein Name
Actin, cytoplasmic type 5
Protein Family
UniProt Entry Name
ACT5_CHICK
NCBI Protein Information
actin, cytoplasmic type 5; gamma-actin; cytoplasmic 2; gamma 1 propeptide; actin, cytoplasmic 2; actin, gamma 1 propeptide; actin, cytoplasmic, type 5

UniProt Comments for ACTG1

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Oxidation of Met-45 and Met-48 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization

By similarity.

Sequence similarities: Belongs to the actin family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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