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ADP Ribosylation Factor Interacting Protein 2 (ARFIP2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630677

Arfaptin 2, or ADP-ribosylation factor interacting protein 2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia.1 Arfaptin-2 has been shown to be involved in… More details

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Product Overview

Online Datasheet in PDF
Product Name
ADP Ribosylation Factor Interacting Protein 2 (ARFIP2), Polyclonal Antibody
Full Product Name
ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1)
Product Gene Name
Product Synonym Names
Anti -ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as a liquid in PBS, pH7.4, 0.1% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Specificity
Recognizes human Arfaptin 2 protein at ~38kD. Also bands observed at ~23, ~35, and ~150kD in some lysate samples. Species Crossreactivity: rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
37,856 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (6496910..6502595, complement). Location: 11p15
OMIM
601638
3D Structure
ModBase 3D Structure for P53365
Immunogen
Synthetic peptide derived from the N-terminal region of the human Arfaptin 2 protein (ADP-ribosylation factor interacting protein 2, AFP2, Partner of Rac1, or POR1)
Positive Control
human HeLa cervical carcinoma, A549 lung carcinoma, MCF-7 breast adenocarcinoma, T47D breast ductal carcinoma, NCI-H69 lung carcinoma, rat PC12, and GFP-Arfaptin 2-transfected HeLa cell lysates.
Applications Tested/Suitable for anti-ARFIP2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-ARFIP2 antibody
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 0.3-7ug/ml
Western Blot: 1-3ug/ml

Other Notes

Small volumes of anti-ARFIP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARFIP2 purchase

MBS630677 is an antibody targeting ARFIP2. Also known as ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1). Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARFIP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
ADP-ribosylation factor interacting protein 2
NCBI Official Synonym Full Names
ADP-ribosylation factor interacting protein 2
NCBI Official Symbol
ARFIP2
NCBI Official Synonym Symbols
POR1; FLJ18046; FLJ18697; FLJ99239
UniProt Protein Name
Arfaptin-2
UniProt Synonym Protein Names
ADP-ribosylation factor-interacting protein 2; Partner of RAC1
Protein Family
UniProt Gene Name
ARFIP2
UniProt Synonym Gene Names
POR1
UniProt Entry Name
ARFP2_HUMAN
NCBI Protein Information
arfaptin-2; OTTHUMP00000164590; OTTHUMP00000231513; partner of RAC1 (arfaptin 2)

UniProt Comments for ARFIP2

arfaptin 2: Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: ruffle; cytoplasm; plasma membrane; trans-Golgi network membrane; cell cortex

Molecular Function: protein domain specific binding; protein binding; GTP binding; GTP-dependent protein binding; Rac GTPase binding

Biological Process: lamellipodium biogenesis; small GTPase mediated signal transduction; ruffle organization and biogenesis; actin cytoskeleton organization and biogenesis; cell motility
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ARFIP2 antibody

 Products by Pathway  Pathway Diagram
 Arf1 Pathway antibodies  Arf1 Pathway Diagram

Diseases associated with anti-ARFIP2 antibody

Organs/Tissues associated with anti-ARFIP2 antibody

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