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BLK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630698

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in… More details

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Sample Manual Insert View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BLK, Polyclonal Antibody
Full Product Name
BLK, NT (Tyrosine-protein Kinase Blk, B Lymphoid Tyrosine Kinase, p55-Blk)
Product Gene Name
anti-BLK antibody
Product Synonym Names
Anti -BLK, NT (Tyrosine-protein Kinase Blk, B Lymphoid Tyrosine Kinase, p55-Blk)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human BLK. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
67,424 Da
Chromosome Location
Chromosome: 8; NC_000008.10 (11351521..11422108). Location: 8p23-p22
3D Structure
ModBase 3D Structure for Q05D26
Immunogen
Synthetic peptide selected from the N-terminal region of human BLK (KLH).

Product Categories/Family for anti-BLK antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-BLK antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-BLK antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-BLK antibody

Western Blot analysis of MBS630698 in HL-60 cell line lysates (35ug/lane).This demonstrates the BLK antibody detected the BLK protein (arrow).
Rabbit Anti-BLK Polyclonal Antibody – Cat# MBS630698

Western Blot (WB) of anti-BLK antibody

Western Blot analysis of MBS630698 in mouse kidney tissue lysate. BLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Rabbit Anti-BLK Polyclonal Antibody, Western Blot (WB) – Cat# MBS630698

Western Blot (WB) of anti-BLK antibody

Western Blot analysis of MBS630698 transiently transfected HEK-293 cell line lysate (1ug/lane). BLK pab(arrow) was detected using the purified Pab.
Rabbit Anti-BLK Polyclonal Antibody, Western Blot (WB) – Cat# MBS630698

Immunohistochemistry (IHC) of anti-BLK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS630698, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-BLK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630698

Other Notes

Small volumes of anti-BLK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BLK purchase

MBS630698 is an antibody targeting BLK. Also known as BLK, NT (Tyrosine-protein Kinase Blk, B Lymphoid Tyrosine Kinase, p55-Blk). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BLK. It may not necessarily be applicable to this product.

NCBI GI #
116284012
NCBI GeneID
640
UniProt Primary Accession #
Q05D26
UniProt Related Accession #
P51451; Q05D26; Q96IN1
NCBI Official Full Name
BLK protein
NCBI Official Synonym Full Names
B lymphoid tyrosine kinase
NCBI Official Symbol
BLK
NCBI Official Synonym Symbols
MODY11; MGC10442
UniProt Protein Name
BLK protein
Protein Family
Tyrosine-protein kinase
UniProt Gene Name
BLK
UniProt Entry Name
Q05D26_HUMAN
NCBI Protein Information
tyrosine-protein kinase Blk; p55-Blk; OTTHUMP00000115909; OTTHUMP00000229490; b lymphocyte kinase; BLK nonreceptor tyrosine kinase

NCBI Summary for BLK

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq]
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UniProt Comments for BLK

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; innate immune response; positive regulation of insulin secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation; T cell differentiation

Disease: Maturity-onset Diabetes Of The Young, Type 11
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Research Articles on BLK

1. study evaluated SNP rs2248932 from BLK and further defined its role in systemic lupus erythematosus (SLE) risk; its association with SLE was confirmed in Chinese Han population

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BLK antibody

 Reference Product  PubMed Publications
 BCR antibody  >15 publications with BLK and BCR
 CD79A antibody  >5 publications with BLK and CD79A
 CD79B antibody  >4 publications with BLK and CD79B
 FCGR2B antibody  >1 publications with BLK and FCGR2B
 FCGR2A antibody  >1 publications with BLK and FCGR2A
 CBL antibody  >1 publications with BLK and CBL
 BCL2 antibody  >1 publications with BLK and BCL2

Pathways associated with anti-BLK antibody

 Products by Pathway  Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Class I PI3K Signaling Events Pathway antibodies  Class I PI3K Signaling Events Pathway Diagram
 EPHA Forward Signaling Pathway antibodies  EPHA Forward Signaling Pathway Diagram
 Ephrin B Reverse Signaling Pathway antibodies  Ephrin B Reverse Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Glypican 1 Network Pathway antibodies  Glypican 1 Network Pathway Diagram
 IL-7 Signaling Pathway antibodies  IL-7 Signaling Pathway Diagram
 PDGFR-beta Signaling Pathway antibodies  PDGFR-beta Signaling Pathway Diagram

Diseases associated with anti-BLK antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >49 publications with BLK and Neoplasms
 Lupus Erythematosus, Systemic Antibodies  >24 publications with BLK and Lupus Erythematosus, Systemic
 Skin Diseases Antibodies  >18 publications with BLK and Skin Diseases
 Lymphoma Antibodies  >15 publications with BLK and Lymphoma
 Vascular Diseases Antibodies  >14 publications with BLK and Vascular Diseases
 Leukemia Antibodies  >13 publications with BLK and Leukemia
 Cardiovascular Diseases Antibodies  >12 publications with BLK and Cardiovascular Diseases
 Lymphoma, B-Cell Antibodies  >12 publications with BLK and Lymphoma, B-Cell
 Arthritis, Rheumatoid Antibodies  >9 publications with BLK and Arthritis, Rheumatoid
 Ischemia Antibodies  >7 publications with BLK and Ischemia

Organs/Tissues associated with anti-BLK antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >118 publications with BLK and Blood
 Spleen Antibodies  >15 publications with BLK and Spleen
 Lung Antibodies  >10 publications with BLK and Lung
 Bone Marrow Antibodies  >8 publications with BLK and Bone Marrow
 Lymph Node Antibodies  >4 publications with BLK and Lymph Node
 Lymph Antibodies  >4 publications with BLK and Lymph
 Thymus Antibodies  >3 publications with BLK and Thymus
 Intestine Antibodies  >3 publications with BLK and Intestine
 Connective Tissue Antibodies  >3 publications with BLK and Connective Tissue
 Pancreas Antibodies  >2 publications with BLK and Pancreas
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