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MSK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630705

MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress (1). MSK1 resembles RSK by having two kinase domains connected by a regulatory linker region (2). Phosphorylation of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MSK1, Polyclonal Antibody
Full Product Name
MSK1, phosphorylated (Ser376) (Mitogen and Stress activated protein Kinase1)
Product Gene Name
Product Synonym Names
Anti -MSK1, phosphorylated (Ser376) (Mitogen and Stress activated protein Kinase1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Detects endogenous MSK1 only when phosphorylated at Ser376. Does not recognize MSK1 phosphorylated at other sites, nor does it recognize phosphorylated RSK1, RSK2 or RSK3.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
66,128 Da
3D Structure
ModBase 3D Structure for P32048
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser376 of human MSK1
Applications Tested/Suitable for anti-MSK1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-MSK1 antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

Western Blot (WB) of anti-MSK1 antibody

Western blot analysis of extracts from RSK1, RSK2, RSK3 or MSK1 transfected 293 cells, untreated or TPA-treated (200 nM), using MBS630705
Rabbit Anti-MSK1 Polyclonal Antibody – Cat# MBS630705

Western Blot (WB) of anti-MSK1 antibody

Western blot analysis of extracts from 293 cells, untreated, TPA-treated or UV-treated, using MBS630705.
Rabbit Anti-MSK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630705

Western Blot (WB) of anti-MSK1 antibody

Western blot analysis of extracts from 293 cells transfected with MSK1 or various mutants of MSK1, then treated with TPA (200 nM), and analyzed using MBS630705
Rabbit Anti-MSK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630705

Other Notes

Small volumes of anti-MSK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSK1 purchase

MBS630705 is an antibody targeting MSK1. Also known as MSK1, phosphorylated (Ser376) (Mitogen and Stress activated protein Kinase1). Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MSK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
MSK1
UniProt Protein Name
Lysine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names
Lysyl-tRNA synthetase
UniProt Gene Name
MSK1
UniProt Synonym Gene Names
LysRS
UniProt Entry Name
SYKM_YEAST

UniProt Comments for MSK1

Catalytic activity: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Subcellular location: Mitochondrion matrix.

Miscellaneous: Present with 2280 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the class-II aminoacyl-tRNA synthetase family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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