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ROCK-1 (rok) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630721

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROK alpha/ROCK-II and ROK beta/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and… More details

$725 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ROCK-1 (rok), Polyclonal Antibody
Full Product Name
ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1, ROCK1, MGC131603, MGC43611, NY-REN-35 Antigen, p160 ROCK1, p160ROCK, Renal Carcinoma Antigen NY-REN-35, Rho-associated Protein Kinase 1)
Product Gene Name
Product Synonym Names
Anti -ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1, ROCK1, MGC131603, MGC43611, NY-REN-35 Antigen, p160 ROCK1, p160ROCK, Renal Carcinoma Antigen NY-REN-35, Rho-associated Protein Kinase 1)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM socium chloride, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ROCK-1. Species Crossreactiviy: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
160,307 Da
Chromosome Location
Chromosome: X; NC_004354.3 (16519142..16531452, complement). Location: 14F2-14F4
3D Structure
ModBase 3D Structure for Q9VXE3
Immunogen
Synthetic peptide corresponding to human ROCK-1.
Positive Control
A431 cell lysate
Applications Tested/Suitable for anti-rok antibody
Western Blot (WB)
Application Notes for anti-rok antibody
Suitable for use in Western Blot.
Dilution: Western Blot: 1:500

Western Blot (WB) of anti-rok antibody

Western Blot Analysis: Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-ROCK-1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates ROCK-1 (~ 158 kDa).
Rabbit Anti-rok Polyclonal Antibody – Cat# MBS630721

Other Notes

Small volumes of anti-rok antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rok purchase

MBS630721 is an antibody targeting rok. Also known as ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1, ROCK1, MGC131603, MGC43611, NY-REN-35 Antigen, p160 ROCK1, p160ROCK, Renal Carcinoma Antigen NY-REN-35, Rho-associated Protein Kinase 1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for rok. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Rho-kinase
NCBI Official Synonym Full Names
Rho-kinase
NCBI Official Symbol
rok
NCBI Official Synonym Symbols
CG9774; DmelCG9774; DRhk; drok; dRok; dROK; Drok; DRok; DROK; Rhk; RhoK; Rock; ROCK; ROCK-1; ROCK1; Rok; ROK; ROKalpha/beta
UniProt Protein Name
FI04037p
Protein Family
UniProt Gene Name
rok
UniProt Synonym Gene Names
rok-RA
UniProt Entry Name
Q9VXE3_DROME
NCBI Protein Information
CG9774 gene product from transcript CG9774-RA; CG9774-PA; DRho-kinase; ROCK; Rho-associated kinase; RhoA kinase; drosophila rho-associated kinase; rho kinase; rok-PA

UniProt Comments for rok

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. SAAS SAAS002219

Sequence similarities: Contains AGC-kinase C-terminal domain. SAAS SAAS002219Contains PH domain. SAAS SAAS002219Contains protein kinase domain. SAAS SAAS002219

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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