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HMGB3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6307276

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HMGB3, Polyclonal Antibody
Full Product Name
HMGB3, ID (HMGB3, HMG2A, HMG4, High mobility group protein B3, High mobility group protein 2a, High mobility group protein 4) (APC)
Product Gene Name
Product Synonym Names
HMGB3; HMG2A; HMG4; High mobility group protein B3; High mobility group protein 2a; High mobility group protein 4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
22,980 Da
Chromosome Location
Chromosome: X; NC_000023.10 (150151763..150159248). Location: Xq28
OMIM
gene 300915
3D Structure
ModBase 3D Structure for O15347
Immunogen
HMGB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the Central region of human HMGB3.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Applications Tested/Suitable for anti-HMGB3 antibody
Western Blot (WB), FLISA
Application Notes for anti-HMGB3 antibody
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-HMGB3 antibody

HMGB3 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HMGB3 antibody detected the HMGB3 protein (arrow).
Rabbit Anti-HMGB3 Polyclonal Antibody – Cat# MBS6307276

Product References and Citations for anti-HMGB3 antibody

Nemeth, M.J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(37):13783-13788(2006). Ross, M.T., et al. Nature 434(7031):325-337(2005). Vaccari, T., et al. Genomics 49(2):247-252(1998). Wilke, K., et al. Gene 198 (1-2), 269-274 (1997): Davis, D.L., et al. Gene 113(2):251-256(1992).

Other Notes

Small volumes of anti-HMGB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HMGB3 purchase

MBS6307276 is an antibody targeting HMGB3. Also known as HMGB3, ID (HMGB3, HMG2A, HMG4, High mobility group protein B3, High mobility group protein 2a, High mobility group protein 4) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HMGB3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
high mobility group protein B3
NCBI Official Synonym Full Names
high mobility group box 3
NCBI Official Symbol
HMGB3
NCBI Official Synonym Symbols
HMG4; HMG-4; HMG2A; HMG-2a
UniProt Protein Name
High mobility group protein B3
UniProt Synonym Protein Names
High mobility group protein 2a; HMG-2a; High mobility group protein 4; HMG-4
UniProt Gene Name
HMGB3
UniProt Synonym Gene Names
HMG2A; HMG4; HMG-2a; HMG-4
UniProt Entry Name
HMGB3_HUMAN
NCBI Protein Information
high mobility group protein B3; high-mobility group box 3; high mobility group protein 4; high mobility group protein 2a; non-histone chromosomal protein; high-mobility group (nonhistone chromosomal) protein 4

UniProt Comments for HMGB3

HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: chromosome; nucleus

Molecular Function: double-stranded DNA binding; chromatin binding; DNA bending activity

Biological Process: chromatin remodeling; negative regulation of B cell differentiation; negative regulation of myeloid cell differentiation; regulation of transcription, DNA-dependent; multicellular organismal development; DNA recombination

Disease: Microphthalmia, Syndromic 13
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HMGB3 antibody

 Reference Product  PubMed Publications
 HMGA1 antibody  >1 publications with HMGB3 and HMGA1
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