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PFKFB1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630732

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in… More details

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Product Overview

Online Datasheet in PDF
Product Name
PFKFB1, Polyclonal Antibody
Full Product Name
PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1, 6PF-2-K/Fru-2,6-P2ase 1, PFK/FBPase 1, 6PF-2-K/Fru-2,6-P2ase Liver Isozyme, F6PK, HL2K, MGC116715, MGC116717, PFRX)
Product Gene Name
anti-PFKFB1 antibody
Product Synonym Names
Anti -PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1, 6PF-2-K/Fru-2,6-P2ase 1, PFK/FBPase 1, 6PF-2-K/Fru-2,6-P2ase Liver Isozyme, F6PK, HL2K, MGC116715, MGC116717, PFRX)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PFKFB1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
31,422 Da
Chromosome Location
Chromosome: X; NC_000023.10 (54959590..55020511, complement). Location: Xp11.21
3D Structure
ModBase 3D Structure for Q4VBA9
Immunogen
Synthetic peptide selected from the center region of human PFKFB1 (KLH).

Product Categories/Family for anti-PFKFB1 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-PFKFB1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PFKFB1 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Immunohistochemistry (IHC) of anti-PFKFB1 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PFKFB1 Polyclonal Antibody – Cat# MBS630732

Western Blot (WB) of anti-PFKFB1 antibody

Western blot analysis of anti-PFKFB1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). PFKFB1(arrow) was detected using the purified Pab.
Rabbit Anti-PFKFB1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630732

Other Notes

Small volumes of anti-PFKFB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFKFB1 purchase

MBS630732 is an antibody targeting PFKFB1. Also known as PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1, 6PF-2-K/Fru-2,6-P2ase 1, PFK/FBPase 1, 6PF-2-K/Fru-2,6-P2ase Liver Isozyme, F6PK, HL2K, MGC116715, MGC116717, PFRX). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PFKFB1. It may not necessarily be applicable to this product.

NCBI GI #
64654084
NCBI GeneID
5207
UniProt Primary Accession #
Q4VBA9
UniProt Related Accession #
P16118; Q4VBA8; Q4VBA9
NCBI Official Full Name
PFKFB1 protein
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
NCBI Official Symbol
PFKFB1
NCBI Official Synonym Symbols
F6PK; HL2K; PFRX; MGC116715; MGC116717
UniProt Protein Name
PFKFB1 protein
Protein Family
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
UniProt Gene Name
PFKFB1
UniProt Entry Name
Q4VBA9_HUMAN
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1; PFK/FBPase 1; OTTHUMP00000023391; 6PF-2-K/Fru-2,6-P2ase 1; 6PF-2-K/Fru-2,6-P2ASE liver isozyme; fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase

NCBI Summary for PFKFB1

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq]
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UniProt Comments for PFKFB1

PFKFB1: Synthesis and degradation of fructose 2,6-bisphosphate. Homodimer. Liver. Phosphorylation results in inhibition of the kinase activity.

Protein type: EC 2.7.1.105; Carbohydrate Metabolism - fructose and mannose; Phosphatase (non-protein); Kinase, other; EC 3.1.3.46; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 complex; cytosol

Molecular Function: identical protein binding; protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; kinase binding; ATP binding

Biological Process: organ regeneration; response to glucagon stimulus; glycolysis; response to cAMP; response to glucocorticoid stimulus; glucose metabolic process; pathogenesis; fructose 2,6-bisphosphate metabolic process; response to insulin stimulus; gluconeogenesis; response to starvation; dephosphorylation; carbohydrate phosphorylation; carbohydrate metabolic process; energy reserve metabolic process; positive regulation of glucokinase activity; fructose metabolic process
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Research Articles on PFKFB1

1. Observational study of genetic testing. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PFKFB1 antibody

 Products by Pathway  Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Fructose And Mannose Metabolism Pathway antibodies  Fructose And Mannose Metabolism Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram
 PKA-mediated Phosphorylation Of Key Metabolic Factors Pathway antibodies  PKA-mediated Phosphorylation Of Key Metabolic Factors Pathway Diagram
 PP2A-mediated Dephosphorylation Of Key Metabolic Factors Pathway antibodies  PP2A-mediated Dephosphorylation Of Key Metabolic Factors Pathway Diagram

Diseases associated with anti-PFKFB1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7 publications with PFKFB1 and Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with PFKFB1 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >1 publications with PFKFB1 and Neoplasms, Experimental
 Obesity Antibodies  >1 publications with PFKFB1 and Obesity
 Carcinoma Antibodies  >1 publications with PFKFB1 and Carcinoma
 Adenocarcinoma Antibodies  >1 publications with PFKFB1 and Adenocarcinoma
 Weight Gain Antibodies  >1 publications with PFKFB1 and Weight Gain
 Nervous System Diseases Antibodies  >1 publications with PFKFB1 and Nervous System Diseases
 Drug Toxicity Antibodies  >1 publications with PFKFB1 and Drug Toxicity
 Brain Diseases Antibodies  >1 publications with PFKFB1 and Brain Diseases

Organs/Tissues associated with anti-PFKFB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >7 publications with PFKFB1 and Liver
 Muscle Antibodies  >4 publications with PFKFB1 and Muscle
 Heart Antibodies  >2 publications with PFKFB1 and Heart
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