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SRSF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630747

SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress… More details

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Product Overview

Online Datasheet in PDF
Product Name
SFRS1 (SRSF1), Polyclonal Antibody
Full Product Name
SFRS1, CT (Splicing Factor, Arginine/Serine-rich 1, pre-mRNA-splicing Factor SF2, P33 Subunit, Alternative-splicing Factor 1, ASF-1, OK/SW-cl.3, SF2P33, SF2, ASF)
Product Gene Name
anti-SRSF1 antibody
Product Synonym Names
Anti -SFRS1, CT (Splicing Factor, Arginine/Serine-rich 1, pre-mRNA-splicing Factor SF2, P33 Subunit, Alternative-splicing Factor 1, ASF-1, OK/SW-cl.3, SF2P33, SF2, ASF)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human SFRS1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
27,745 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (56078280..56084707, complement). Location: 17q22
OMIM
600812
3D Structure
ModBase 3D Structure for Q07955
Immunogen
Synthetic peptide selected from the C-terminal region of human SFRS1 (KLH).

Product Categories/Family for anti-SRSF1 antibody

Antibodies; Abs to Splicing Factors
Applications Tested/Suitable for anti-SRSF1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SRSF1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-SRSF1 antibody

Western blot analysis of SFRS1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.
Rabbit Anti-SRSF1 Polyclonal Antibody – Cat# MBS630747

Immunohistochemistry (IHC) of anti-SRSF1 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-SRSF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630747

Western Blot (WB) of anti-SRSF1 antibody

Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.
Rabbit Anti-SRSF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630747

Flow Cytometry (FC/FACS) of anti-SRSF1 antibody

SFRS1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SRSF1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS630747

Other Notes

Small volumes of anti-SRSF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SRSF1 purchase

MBS630747 is an antibody targeting SRSF1. Also known as SFRS1, CT (Splicing Factor, Arginine/Serine-rich 1, pre-mRNA-splicing Factor SF2, P33 Subunit, Alternative-splicing Factor 1, ASF-1, OK/SW-cl.3, SF2P33, SF2, ASF). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SRSF1. It may not necessarily be applicable to this product.

NCBI GI #
45708738
NCBI GeneID
6426
UniProt Primary Accession #
Q07955
UniProt Secondary Accession #
Q13809; B2R6Z7; D3DTZ3
UniProt Related Accession #
Q07955; Q59FA2
NCBI Official Full Name
SFRS1 protein
NCBI Official Synonym Full Names
serine/arginine-rich splicing factor 1
NCBI Official Symbol
SRSF1
NCBI Official Synonym Symbols
ASF; SF2; SFRS1; SF2p33; SRp30a; MGC5228; FLJ53078
UniProt Protein Name
Serine/arginine-rich splicing factor 1
UniProt Synonym Protein Names
Alternative-splicing factor 1; ASF-1; Splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit
Protein Family
Serine/arginine-rich splicing factor
UniProt Gene Name
SRSF1
UniProt Synonym Gene Names
ASF; SF2; SF2P33; SFRS1
UniProt Entry Name
SRSF1_HUMAN
NCBI Protein Information
serine/arginine-rich splicing factor 1; ASF-1; splicing factor 2; SR splicing factor 1; alternative-splicing factor 1; splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit

NCBI Summary for SRSF1

This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 13. [provided by RefSeq]
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UniProt Comments for SRSF1

SF2: a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5' and 3' splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: mRNA binding; RS domain binding; protein binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; RNA splicing; regulation of mRNA stability; nuclear mRNA 5'-splice site recognition; nuclear mRNA splicing, via spliceosome; regulation of translation; mRNA splice site selection; mRNA export from nucleus; regulation of nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction
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Research Articles on SRSF1

1. Findings show that oncogenic factors activating the phosphoinositide 3-kinase/Akt pathway can regulate alternative splicing of Casp9 via a coordinated mechanism involving the phosphorylation of SRp30a.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SRSF1 antibody

 Reference Product  PubMed Publications
 SRPK1 antibody  >3 publications with SRSF1 and SRPK1

Pathways associated with anti-SRSF1 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Formation And Maturation Of MRNA Transcript Pathway antibodies  Formation And Maturation Of MRNA Transcript Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Transcription Pathway antibodies  Transcription Pathway Diagram
 Transport Of Mature Transcript To Cytoplasm Pathway antibodies  Transport Of Mature Transcript To Cytoplasm Pathway Diagram

Diseases associated with anti-SRSF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4 publications with SRSF1 and Neoplasms
 Carcinoma Antibodies  >3 publications with SRSF1 and Carcinoma
 Lung Neoplasms Antibodies  >2 publications with SRSF1 and Lung Neoplasms
 Nervous System Diseases Antibodies  >1 publications with SRSF1 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >1 publications with SRSF1 and Cardiovascular Diseases
 Adenoma Antibodies  >1 publications with SRSF1 and Adenoma
 Kidney Neoplasms Antibodies  >1 publications with SRSF1 and Kidney Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with SRSF1 and Neoplasms, Experimental
 Gastrointestinal Diseases Antibodies  >1 publications with SRSF1 and Gastrointestinal Diseases
 Atrophy Antibodies  >1 publications with SRSF1 and Atrophy

Organs/Tissues associated with anti-SRSF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with SRSF1 and Lung
 Heart Antibodies  >1 publications with SRSF1 and Heart
 Intestine Antibodies  >1 publications with SRSF1 and Intestine
 Brain Antibodies  >1 publications with SRSF1 and Brain
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