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UBE4A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630821

Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
UBE4A, Polyclonal Antibody
Full Product Name
UBE4A, CT (Ubiquitin Conjugation Factor E4 A, KIAA0126)
Product Gene Name
anti-UBE4A antibody
Product Synonym Names
Anti -UBE4A, CT (Ubiquitin Conjugation Factor E4 A, KIAA0126)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human UBE4A.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
122,561 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (118230302..118269926). Location: 11q23.3
OMIM
603753
3D Structure
ModBase 3D Structure for Q14139
Immunogen
Synthetic peptide selected from the C-terminal region of human UBE4A (KLH).

Product Categories/Family for anti-UBE4A antibody

Antibodies; Abs to Ubiquitin
Applications Tested/Suitable for anti-UBE4A antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-UBE4A antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:200
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-UBE4A antibody

Western blot analysis of anti-UBE4A Antibody (C-term) in NCI-H460 and CEM cell line lysates (35ug/lane). UBE4A (arrow) was detected using the purified Pab.
Rabbit Anti-UBE4A Polyclonal Antibody – Cat# MBS630821

Immunohistochemistry (IHC) of anti-UBE4A antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-UBE4A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630821

Other Notes

Small volumes of anti-UBE4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE4A purchase

MBS630821 is an antibody targeting UBE4A. Also known as UBE4A, CT (Ubiquitin Conjugation Factor E4 A, KIAA0126). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE4A. It may not necessarily be applicable to this product.

NCBI GI #
323362983
NCBI GeneID
9354
NCBI Accession #
NP_001191006.1
UniProt Primary Accession #
Q14139
UniProt Secondary Accession #
Q2M1H0; Q6P5T4; Q7Z639; B0YJB6
UniProt Related Accession #
Q05BZ7; Q14139
GenBank Nucleotide #
NM_001204077.1
NCBI Official Full Name
ubiquitin conjugation factor E4 A isoform 2
NCBI Official Synonym Full Names
ubiquitination factor E4A
NCBI Official Symbol
UBE4A
NCBI Official Synonym Symbols
E4; UFD2; UBOX2; KIAA0126; MGC133315
UniProt Protein Name
Ubiquitin conjugation factor E4 A
Protein Family
Ubiquitin conjugation factor
UniProt Gene Name
UBE4A
UniProt Synonym Gene Names
KIAA0126
UniProt Entry Name
UBE4A_HUMAN
NCBI Protein Information
ubiquitin conjugation factor E4 A; OTTHUMP00000237916; OTTHUMP00000237917; ubiquitination factor E4A (UFD2 homolog, yeast)

NCBI Summary for UBE4A

This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq]
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UniProt Comments for UBE4A

UBE4A: Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. Belongs to the ubiquitin conjugation factor E4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; ubiquitin ligase complex

Molecular Function: protein binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein polyubiquitination
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Research Articles on UBE4A

1. Identified an intestinal cDNA clone encoding ubiquitination factor E4A (UBE4A), a U-box-type ubiquitin-protein ligase. The prevalence of anti-UBE4A IgG in patients with CD was significantly higher than that in patients with UC or healthy controls

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-UBE4A antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-UBE4A antibody

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >1 publications with UBE4A and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-UBE4A antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with UBE4A and Blood
 Liver Antibodies  >1 publications with UBE4A and Liver
 Intestine Antibodies  >1 publications with UBE4A and Intestine
 Heart Antibodies  >1 publications with UBE4A and Heart
 Muscle Antibodies  >1 publications with UBE4A and Muscle
 Kidney Antibodies  >1 publications with UBE4A and Kidney
 Lung Antibodies  >1 publications with UBE4A and Lung
 Brain Antibodies  >1 publications with UBE4A and Brain
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