Chicken Anti-NEFL Polyclonal Antibody – Cat# MBS630864

Dissociated cell culture prepared from an adult rat brain. NF-H (green staining) can be found within the neuronal cell body and in the neurites using MBS630864. Picture courtesy of Dr. Gerry Shaw (University of Florida).

Neurofilament, Heavy Chain (NEFL) Antibody – Chicken Polyclonal

Name: NEFL Antibody; Polyclonal; Chicken; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS630864
Price: 770 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630864

Human Neurofilament Heavy Chain (NF-H) is a 115.378kD protein (1072aa) that forms part of the neuronal cytoskeleton. NF-H is physically associated with 10nm intermediate filaments, and is said to be one of the "neurofilament triplet" proteins, although it… More details

$770 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Neurofilament, Heavy Chain (NEFL), Polyclonal Antibody

Full Product Name

Neurofilament, Heavy Chain (NF-H)

Product Gene Name

anti-NEFL antibody

Product Synonym Names

Anti -Neurofilament, Heavy Chain (NF-H)

Clonality

Polyclonal

Isotype

IgY

Host

Chicken

Species Reactivity

Rat

Form/Format

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide.

Purity/Purification

Purified
Purified

Specificity

Recognizes Neurofilament, Heavy Chain. Species Crossreactivity: rat.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

61,517 Da

Chromosome Location

Chromosome: 8; NC_000008.10 (24808468..24814383, complement). Location: 8p21

OMIM

162280

3D Structure

ModBase 3D Structure for P07196

Immunogen

Purified neurofilament protein (200kD) isolated from bovine brains. Species sequence homology: Human, mouse.

Product Categories/Family for anti-NEFL antibody

Antibodies; Abs to Neurofilament

Applications Tested/Suitable for anti-NEFL antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Application Notes for anti-NEFL antibody

Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot.
Dilution: Immunohistochemistry: 1:2000:1:5000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:2000:1:5000 using 2% paraformaldehyde cells
Western Blot: 1:5000-1:10,000

Other Notes

Small volumes of anti-NEFL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEFL purchase

MBS630864 is an antibody targeting NEFL. Also known as Neurofilament, Heavy Chain (NF-H). Reactive in Rat. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for NEFL. It may not necessarily be applicable to this product.

NCBI GI #

105990539

NCBI GeneID

4747

NCBI Accession #

NP_006149.2

UniProt Primary Accession #

P07196

UniProt Secondary Accession #

Q16154; Q8IU72; B9ZVN2

UniProt Related Accession #

P07196

GenBank Nucleotide #

NM_006158.4

NCBI Official Full Name

neurofilament light polypeptide

NCBI Official Synonym Full Names

neurofilament, light polypeptide

NCBI Official Symbol

NEFL

NCBI Official Synonym Symbols

NFL; NF-L; NF68; CMT1F; CMT2E

UniProt Protein Name

Neurofilament light polypeptide

UniProt Synonym Protein Names

68 kDa neurofilament protein; Neurofilament triplet L protein

Protein Family

Neurofilament light polypeptide

UniProt Gene Name

NEFL

UniProt Synonym Gene Names

NF68; NFL; NF-L

UniProt Entry Name

NFL_HUMAN

NCBI Protein Information

neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein

NCBI Summary for NEFL

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

UniProt Comments for NEFL

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: TSC1-TSC2 complex; growth cone; axon; cytoplasm; neurofilament; cytosol

Molecular Function: protein C-terminus binding; protein binding, bridging; identical protein binding; protein domain specific binding; protein binding; phospholipase binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; response to peptide hormone stimulus; neurofilament bundle assembly; response to toxin; intermediate filament organization; microtubule cytoskeleton organization and biogenesis; retrograde axon cargo transport; axon regeneration in the peripheral nervous system; synaptic transmission; axon transport of mitochondrion; regulation of axon diameter; positive regulation of axonogenesis; negative regulation of neuron apoptosis; locomotion; neuromuscular process controlling balance; anterograde axon cargo transport; neurite morphogenesis; response to corticosterone stimulus

Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f

Research Articles on NEFL

1. High CSF NFL levels were found in patients with amyotrophic lateral sclerosis (

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEFL antibody

Reference Product	PubMed Publications
MTMR2 antibody	>6 publications with NEFL and MTMR2
NEFH antibody	>3 publications with NEFL and NEFH
GAN antibody	>2 publications with NEFL and GAN
TSC1 antibody	>1 publications with NEFL and TSC1

Pathways associated with anti-NEFL antibody

Products by Pathway	Pathway Diagram
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies	Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies	CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
CREB Phosphorylation Through The Activation Of Ras Pathway antibodies	CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway antibodies	Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway Diagram
Post NMDA Receptor Activation Events Pathway antibodies	Post NMDA Receptor Activation Events Pathway Diagram
Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway antibodies	Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway Diagram
Transmission Across Chemical Synapses Pathway antibodies	Transmission Across Chemical Synapses Pathway Diagram

Diseases associated with anti-NEFL antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>45 publications with NEFL and Nervous System Diseases
Peripheral Nervous System Diseases Antibodies	>38 publications with NEFL and Peripheral Nervous System Diseases
Nervous System Malformations Antibodies	>36 publications with NEFL and Nervous System Malformations
Polyneuropathies Antibodies	>36 publications with NEFL and Polyneuropathies
Neoplasms Antibodies	>30 publications with NEFL and Neoplasms
Breast Neoplasms Antibodies	>7 publications with NEFL and Breast Neoplasms
Brain Diseases Antibodies	>5 publications with NEFL and Brain Diseases
Charcot-Marie-Tooth disease, Type 1F Antibodies	>4 publications with NEFL and Charcot-Marie-Tooth disease, Type 1F
Pain Antibodies	>3 publications with NEFL and Pain
Ataxia Antibodies	>2 publications with NEFL and Ataxia

Organs/Tissues associated with anti-NEFL antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>32 publications with NEFL and Nerve
Brain Antibodies	>16 publications with NEFL and Brain
Prostate Antibodies	>11 publications with NEFL and Prostate
Spinal Cord Antibodies	>5 publications with NEFL and Spinal Cord
Lung Antibodies	>4 publications with NEFL and Lung
Eye Antibodies	>3 publications with NEFL and Eye
Ganglia Antibodies	>2 publications with NEFL and Ganglia
Kidney Antibodies	>2 publications with NEFL and Kidney
Liver Antibodies	>1 publications with NEFL and Liver
Bone Marrow Antibodies	>1 publications with NEFL and Bone Marrow