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Glyoxalase I (GLO1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630880

The enzyme GLO1 is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. More details

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Product Overview

Online Datasheet in PDF
Product Name
Glyoxalase I (GLO1), Polyclonal Antibody
Full Product Name
Glyoxalase I, NT (GLO1, GLOD1, Glx I, GLYI, Aldoketomutase, Ketone-aldehyde Mutase, Lactoylglutathione Lyase, Methylglyoxalase, S-D-lactoylglutathione Methylglyoxal Lyase)
Product Gene Name
Product Synonym Names
Anti -Glyoxalase I, NT (GLO1, GLOD1, Glx I, GLYI, Aldoketomutase, Ketone-aldehyde Mutase, Lactoylglutathione Lyase, Methylglyoxalase, S-D-lactoylglutathione Methylglyoxal Lyase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human GLO1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
20,778 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (38643701..38670952, complement). Location: 6p21.3-p21.1
OMIM
138750
3D Structure
ModBase 3D Structure for Q04760
Immunogen
Synthetic peptide selected from the N-terminal region of human GLO1 (KLH).
Applications Tested/Suitable for anti-GLO1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-GLO1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-GLO1 antibody

Western blot analysis of GLO1 antibody (N-term) in HL60 cell line lysates (35ug/lane). GLO1 (arrow) was detected using the purified Pab.
Rabbit Anti-GLO1 Polyclonal Antibody – Cat# MBS630880

Immunohistochemistry (IHC) of anti-GLO1 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with GLO1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-GLO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630880

Flow Cytometry (FC/FACS) of anti-GLO1 antibody

Flow cytometric analysis of widr cells using GLO1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-GLO1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS630880

Western Blot (WB) of anti-GLO1 antibody

Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.
Rabbit Anti-GLO1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630880

Western Blot (WB) of anti-GLO1 antibody

Western blot analysis of GLO1 (arrow) using rabbit polyclonal GLO1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GLO1 gene.
Rabbit Anti-GLO1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630880

Other Notes

Small volumes of anti-GLO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GLO1 purchase

MBS630880 is an antibody targeting GLO1. Also known as Glyoxalase I, NT (GLO1, GLOD1, Glx I, GLYI, Aldoketomutase, Ketone-aldehyde Mutase, Lactoylglutathione Lyase, Methylglyoxalase, S-D-lactoylglutathione Methylglyoxal Lyase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GLO1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
glyoxalase I
NCBI Official Synonym Full Names
glyoxalase I
NCBI Official Symbol
GLO1
NCBI Official Synonym Symbols
GLYI; GLOD1
UniProt Protein Name
Lactoylglutathione lyase
UniProt Synonym Protein Names
Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
UniProt Gene Name
GLO1
UniProt Entry Name
LGUL_HUMAN
NCBI Protein Information
lactoylglutathione lyase; glx I; aldoketomutase; methylglyoxalase; OTTHUMP00000016339; ketone-aldehyde mutase; lactoyl glutathione lyase; glyoxalase domain containing 1; S-D-lactoylglutathione methylglyoxal lyase

NCBI Summary for GLO1

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]

UniProt Comments for GLO1

GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.4.1.5; Lyase; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 6p21.3-p21.1

Cellular Component: cytoplasm

Molecular Function: zinc ion binding; lactoylglutathione lyase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; methylglyoxal metabolic process; negative regulation of apoptosis

Disease: Autism
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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