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SEPHS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630906

This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SEPSH2 (SEPHS2), Polyclonal Antibody
Full Product Name
SEPSH2, ID (Selenide, Water Dikinase 2, Selenophosphate Synthetase 2, Selenium Donor Protein 2, SPS2)
Product Gene Name
Product Synonym Names
Anti -SEPSH2, ID (Selenide, Water Dikinase 2, Selenophosphate Synthetase 2, Selenium Donor Protein 2, SPS2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human SEPSH2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,305 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (30454952..30457224, complement). Location: 16p11.2
OMIM
606218
3D Structure
ModBase 3D Structure for Q99611
Immunogen
Synthetic peptide selected from the Center region of human SEPSH2 (KLH).
Applications Tested/Suitable for anti-SEPHS2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SEPHS2 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-SEPHS2 antibody

Western blot analysis of anti-SEPSH2 Pab in HepG2 cell line lysate (35ug/lane). SEPSH2(arrow) was detected using the purified Pab.
Rabbit Anti-SEPHS2 Polyclonal Antibody – Cat# MBS630906

Immunohistochemistry (IHC) of anti-SEPHS2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-SEPHS2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630906

Other Notes

Small volumes of anti-SEPHS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEPHS2 purchase

MBS630906 is an antibody targeting SEPHS2. Also known as SEPSH2, ID (Selenide, Water Dikinase 2, Selenophosphate Synthetase 2, Selenium Donor Protein 2, SPS2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SEPHS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
selenide, water dikinase 2
NCBI Official Synonym Full Names
selenophosphate synthetase 2
NCBI Official Symbol
SEPHS2
NCBI Official Synonym Symbols
SPS2; SPS2b
UniProt Protein Name
Selenide, water dikinase 2
UniProt Synonym Protein Names
Selenium donor protein 2; Selenophosphate synthase 2
UniProt Gene Name
SEPHS2
UniProt Synonym Gene Names
SPS2
UniProt Entry Name
SPS2_HUMAN
NCBI Protein Information
selenide, water dikinase 2; selenium donor protein 2; selenophosphate synthase 2

NCBI Summary for SEPHS2

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. Genes encoding selenocysteine contain a stem-loop secondary structure in their 3' UTR called a selenocysteine insertion sequence (SECIS) element. The protein encoded by this gene contains a selenocysteine residue in its predicted active site. There is a pseudogene for this gene on chromosome 5. [provided by RefSeq, Aug 2013]
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UniProt Comments for SEPHS2

SEPHS2: Synthesizes selenophosphate from selenide and ATP. Belongs to the selenophosphate synthase 1 family. Class I subfamily.

Protein type: Other Amino Acids Metabolism - selenoamino acid; Kinase, other; EC 2.7.9.3

Chromosomal Location of Human Ortholog: 16p11.2

Molecular Function: selenide, water dikinase activity; ATP binding

Biological Process: selenocysteine biosynthetic process; phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SEPHS2 antibody

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