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ID1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6309115

MBS6309115 is an antibody targeting ID1. Also known as ID1, ID (ID1, BHLHB24, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1) (FITC). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ID1, Polyclonal Antibody
Full Product Name
ID1, ID (ID1, BHLHB24, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, Inhibitor of DNA binding 1) (FITC)
Product Gene Name
anti-ID1 antibody
Product Synonym Names
ID1; BHLHB24; ID; DNA-binding protein inhibitor ID-1; Class B basic helix-loop-helix protein 24; Inhibitor of DNA binding 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human ID1. Species Crossreactivity: rat, mouse
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
15,586 Da
OMIM
600349
3D Structure
ModBase 3D Structure for P41134
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 66-93 in the central region of human ID1, UniProt Accession #P41134.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-ID1 antibody

Antibodies; Binding Proteins
Applications Tested/Suitable for anti-ID1 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), FLISA, Flow Cytometry (FC/FACS)
Application Notes for anti-ID1 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ID1 antibody

Western Blot analysis in U251 cell line lysates (35ug/lane) using 036863. This demonstrates that 036863 detected the ID1 protein (arrow).
Rabbit Anti-ID1 Polyclonal Antibody – Cat# MBS6309115

Western Blot (WB) of anti-ID1 antibody

Western Blot analysis in mouse heart tissue lysates (35ug/lane) using 036863. This demonstrates that 036863 (PEI 1:100) detected the ID1 protein (arrow).
Rabbit Anti-ID1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6309115

Immunohistochemistry (IHC) of anti-ID1 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using 036863 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 036863 for immunohistochemistry.
Rabbit Anti-ID1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6309115

Immunofluorescence (IF) of anti-ID1 antibody

Confocal immunofluorescent analysis with U-251MG cell using 036863 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-ID1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6309115

Testing Data of anti-ID1 antibody

Overlay histogram showing HepG2 cells using 036863 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by 036863 (1:25) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488o Istype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Anti-ID1 Polyclonal Antibody, Testing Data – Cat# MBS6309115

Product References and Citations for anti-ID1 antibody

1. Qian, T., et al. Oncogene 29(43):5818-5827(2010). 2. Hamajima, Y., et al. Cell Prolif. 43(5):457-463(2010). 3. Yang, J., et al. Circ. Res. 107(2):252-262(2010). 4. Phi, J.H., et al. J Neurosurg Pediatr 5(6):608-614(2010). 5. Geng, H., et al. Cancer Res. 70(8):3239-3248(2010).

Other Notes

Small volumes of anti-ID1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ID1. It may not necessarily be applicable to this product.

NCBI GI #
31317297
NCBI GeneID
3397
NCBI Accession #
NP_851998.1
NCBI Related Accession #
NP_002156.2
UniProt Primary Accession #
P41134
UniProt Secondary Accession #
O00651; O00652; Q16371; Q16377; Q5TE66; Q5TE67; Q969Z7; Q9H0Z5; Q9H109; A8K537; E1P5L4
UniProt Related Accession #
P41134
GenBank Nucleotide #
NM_181353.2
NCBI Official Full Name
DNA-binding protein inhibitor ID-1 isoform b
NCBI Official Synonym Full Names
inhibitor of DNA binding 1, HLH protein
NCBI Official Symbol
ID1
NCBI Official Synonym Symbols
ID; bHLHb24
UniProt Protein Name
DNA-binding protein inhibitor ID-1
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; Inhibitor of differentiation 1
Protein Family
DNA-binding protein inhibitor
UniProt Gene Name
ID1
UniProt Synonym Gene Names
BHLHB24; ID; bHLHb24
UniProt Entry Name
ID1_HUMAN
NCBI Protein Information
DNA-binding protein inhibitor ID-1

NCBI Summary for ID1

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for ID1

ID1: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nucleoplasm; Golgi apparatus; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription factor activity

Biological Process: circadian rhythm; collagen metabolic process; blood vessel endothelial cell migration; transcription, DNA-dependent; protein destabilization; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; BMP signaling pathway; response to antibiotic; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway; blood vessel morphogenesis; negative regulation of osteoblast differentiation; endothelial cell morphogenesis; angiogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
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Research Articles on ID1

1. Therefore, we identified a tumorigenic role of Id-1 in OS and suggested a potential therapeutic target for OS patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ID1 antibody

 Reference Product  PubMed Publications
 SMAD5 antibody  >46 publications with ID1 and SMAD5
 TCF3 antibody  >32 publications with ID1 and TCF3
 SMAD4 antibody  >23 publications with ID1 and SMAD4
 THBS1 antibody  >9 publications with ID1 and THBS1
 MYOD1 antibody  >8 publications with ID1 and MYOD1
 HDAC1 antibody  >6 publications with ID1 and HDAC1
 SMAD9 antibody  >5 publications with ID1 and SMAD9
 ETS2 antibody  >4 publications with ID1 and ETS2
 CDC20 antibody  >3 publications with ID1 and CDC20
 CEBPB antibody  >1 publications with ID1 and CEBPB

Pathways associated with anti-ID1 antibody

 Products by Pathway  Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 Oncogene Induced Senescence Pathway antibodies  Oncogene Induced Senescence Pathway Diagram

Diseases associated with anti-ID1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >70 publications with ID1 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >61 publications with ID1 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >55 publications with ID1 and Disease Models, Animal
 Lung Neoplasms Antibodies  >46 publications with ID1 and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >41 publications with ID1 and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >40 publications with ID1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >30 publications with ID1 and Liver Diseases
 Cardiovascular Diseases Antibodies  >28 publications with ID1 and Cardiovascular Diseases
 Brain Diseases Antibodies  >27 publications with ID1 and Brain Diseases
 Hypertension Antibodies  >26 publications with ID1 and Hypertension

Organs/Tissues associated with anti-ID1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >283 publications with ID1 and Bone
 Blood Antibodies  >183 publications with ID1 and Blood
 Vascular Antibodies  >128 publications with ID1 and Vascular
 Muscle Antibodies  >106 publications with ID1 and Muscle
 Lung Antibodies  >89 publications with ID1 and Lung
 Brain Antibodies  >84 publications with ID1 and Brain
 Liver Antibodies  >76 publications with ID1 and Liver
 Prostate Antibodies  >65 publications with ID1 and Prostate
 Embryonic Tissue Antibodies  >62 publications with ID1 and Embryonic Tissue
 Skin Antibodies  >40 publications with ID1 and Skin
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