Rabbit Anti-SAE1 Polyclonal Antibody – Cat# MBS630953

Western blot using Immunochemicals’ Rabbit-anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 _g human HeLa whole cell protein. Right lane (+) contains 20 _g human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos- phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.

SUMO-1 Activating Enzyme, Subunit 1 (SAE1) Antibody – Rabbit Polyclonal

Name: SAE1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS630953
Price: 1140 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630953

SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SUMO-1 Activating Enzyme, Subunit 1 (SAE1), Polyclonal Antibody

Full Product Name

SUMO-1 Activating Enzyme, Subunit 1 (SAE1, AOS1, FLJ3091, HSPC140, Sentrin/SUMO-activating Protein AOS1, SUA1, SUMO1 Activating Enzyme E1 N Subunit, Ubiquitin-like 1-activating Enzyme E1A, UBLE1A, Ubiquitin-like Protein SUMO1 Activating Enzyme)

Product Gene Name

anti-SAE1 antibody

Product Synonym Names

Anti -SUMO-1 Activating Enzyme, Subunit 1 (SAE1, AOS1, FLJ3091, HSPC140, Sentrin/SUMO-activating Protein AOS1, SUA1, SUMO1 Activating Enzyme E1 N Subunit, Ubiquitin-like 1-activating Enzyme E1A, UBLE1A, Ubiquitin-like Protein SUMO1 Activating Enzyme)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human. Species Sequence Homology: bovine, dog, chimpanzee(96%), mouse (93%), and rat(92%).

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

~37kD

Chromosome Location

Chromosome: 19; NC_000019.9 (47634080..47713893). Location: 19q13.32

OMIM

613294

3D Structure

ModBase 3D Structure for Q9UBE0

Immunogen

Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein

Product Categories/Family for anti-SAE1 antibody

Antibodies; Abs to Ubiquitin

Applications Tested/Suitable for anti-SAE1 antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-SAE1 antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:100,000-1:125,000
Western Blot: 1:750-1:1,000

Other Notes

Small volumes of anti-SAE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SAE1 purchase

MBS630953 is an antibody targeting SAE1. Also known as SUMO-1 Activating Enzyme, Subunit 1 (SAE1, AOS1, FLJ3091, HSPC140, Sentrin/SUMO-activating Protein AOS1, SUA1, SUMO1 Activating Enzyme E1 N Subunit, Ubiquitin-like 1-activating Enzyme E1A, UBLE1A, Ubiquitin-like Protein SUMO1 Activating Enzyme). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SAE1. It may not necessarily be applicable to this product.

NCBI GI #

4885585

NCBI GeneID

10055

NCBI Accession #

NP_005491.1

UniProt Primary Accession #

Q9UBE0

UniProt Secondary Accession #

O95717; Q9P020; B2RDP5

UniProt Related Accession #

Q9UBE0

GenBank Nucleotide #

NM_005500.2

NCBI Official Full Name

SUMO-activating enzyme subunit 1 isoform a

NCBI Official Synonym Full Names

SUMO1 activating enzyme subunit 1

NCBI Official Symbol

SAE1

NCBI Official Synonym Symbols

AOS1; SUA1; UBLE1A; FLJ3091; HSPC140

UniProt Protein Name

SUMO-activating enzyme subunit 1

UniProt Synonym Protein Names

Ubiquitin-like 1-activating enzyme E1A

Protein Family

SUMO-activating enzyme

UniProt Gene Name

SAE1

UniProt Synonym Gene Names

AOS1; SUA1; UBLE1A

UniProt Entry Name

SAE1_HUMAN

NCBI Protein Information

SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme

NCBI Summary for SAE1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM]

UniProt Comments for SAE1

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus; cytosol

Molecular Function: protein C-terminus binding; ATP-dependent protein binding; SUMO activating enzyme activity; protein binding; protein heterodimerization activity; enzyme activator activity; ubiquitin activating enzyme activity

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; regulation of mitotic cell cycle; protein ubiquitination; SMT3-dependent protein catabolic process; post-translational protein modification

Research Articles on SAE1

1. The mammalian E1 subunits can be imported separately, identify nuclear localization signals (NLSs) in Aos1 and in Uba2, and demonstrate that their import is mediated by importin alpha/beta in vitro and in intact cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SAE1 antibody

Reference Product	PubMed Publications
UBA2 antibody	>11 publications with SAE1 and UBA2
SUMO2 antibody	>3 publications with SAE1 and SUMO2
SUMO3 antibody	>2 publications with SAE1 and SUMO3

Pathways associated with anti-SAE1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immunity Signaling Pathway antibodies	Adaptive Immunity Signaling Pathway Diagram
Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies	Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram
Ubiquitin Mediated Proteolysis Pathway antibodies	Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-SAE1 antibody

Disease Name	Pubmed Publications
Seizures Antibodies	>1 publications with SAE1 and Seizures

Organs/Tissues associated with anti-SAE1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with SAE1 and Brain
Muscle Antibodies	>1 publications with SAE1 and Muscle
Uterus Antibodies	>1 publications with SAE1 and Uterus
Skin Antibodies	>1 publications with SAE1 and Skin