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CD316 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6309607

MBS6309607 is an antibody targeting CD316. Also known as CD316, ID (Immunoglobulin superfamily member 8, CD81 partner 3, Glu-Trp-Ile EWI motif-containing protein 2, EWI-2, Keratinocytes-associated transmembrane protein 4, KCT-4, LIR-D1, IGSF8, CD81P3, EWI2,… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD316, Polyclonal Antibody
Full Product Name
CD316, ID (Immunoglobulin superfamily member 8, CD81 partner 3, Glu-Trp-Ile EWI motif-containing protein 2, EWI-2, Keratinocytes-associated transmembrane protein 4, KCT-4, LIR-D1, IGSF8, CD81P3, EWI2, KCT4) (AP)
Product Gene Name
Product Synonym Gene Name
IGSF8
Product Synonym Names
IGSF8; CD81P3; EWI2; KCT4; Immunoglobulin superfamily member 8; CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; Keratinocytes-associated transmembrane protein 4; LIR-D1; Prostaglandin regulatory-like protein; CD316
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
40,191 Da
3D Structure
ModBase 3D Structure for Q969P0
Immunogen
IGSF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-262 amino acids from the Central region of human IGSF8.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-CD316 antibody

Applications Tested/Suitable for anti-CD316 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-CD316 antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CD316 antibody

IGSF8 Antibody (Center) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the IGSF8 antibody detected the IGSF8 protein (arrow).
Rabbit Anti-CD316 Polyclonal Antibody – Cat# MBS6309607

Immunohistochemistry (IHC) of anti-CD316 antibody

IGSF8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGSF8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-CD316 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6309607

Product References and Citations for anti-CD316 antibody

Kolesnikova, T.V., et al. Neoplasia 11(1):77-86(2009). Rocha-Perugini, V., et al. PLoS ONE 3 (4), E1866 (2008): Kettner, S., et al. Mol. Cell. Biol. 27(21):7718-7726(2007). Sala-Valdes, M., et al. J. Biol. Chem. 281(28):19665-19675(2006). Yang, X.H., et al. J. Biol. Chem. 281(18):12976-12985(2006).

Other Notes

Small volumes of anti-CD316 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD316. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
immunoglobulin superfamily member 8
NCBI Official Synonym Full Names
immunoglobulin superfamily member 8
NCBI Official Symbol
IGSF8
NCBI Official Synonym Symbols
EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
UniProt Protein Name
Immunoglobulin superfamily member 8
UniProt Synonym Protein Names
CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; EWI-2; Keratinocytes-associated transmembrane protein 4; KCT-4; LIR-D1; Prostaglandin regulatory-like protein; PGRL; CD_antigen: CD316
UniProt Gene Name
IGSF8
UniProt Synonym Gene Names
CD81P3; EWI2; KCT4; IgSF8; EWI-2; KCT-4; PGRL
NCBI Protein Information
immunoglobulin superfamily member 8

NCBI Summary for CD316

This gene encodes a member the EWI subfamily of the immunoglobulin protein superfamily. Members of this family contain a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a variable number of immunoglobulin domains. This protein interacts with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. The encoded protein may also function as a tumor suppressor by inhibiting the proliferation of certain cancers. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
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UniProt Comments for CD316

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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