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Cmaq_1224 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630969

RFK (riboflavin kinase) catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN). More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RFK (Cmaq_1224), Polyclonal Antibody
Full Product Name
RFK, NT (Riboflavin Kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase)
Product Gene Name
Product Synonym Names
Anti -RFK, NT (Riboflavin Kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human RFK.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
25,887 Da
Chromosome Location
NC_009954.1 (1294955..1295647)
3D Structure
ModBase 3D Structure for A8ME45
Immunogen
Synthetic peptide selected from the N-terminal region of human RFK (KLH).
Applications Tested/Suitable for anti-Cmaq_1224 antibody
ELISA (EL/EIA), Immunohistochemistry (IHC)
Application Notes for anti-Cmaq_1224 antibody
Suitable for use in ELISA and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100

Immunohistochemistry (IHC) of anti-Cmaq_1224 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-Cmaq_1224 Polyclonal Antibody – Cat# MBS630969

Western Blot (WB) of anti-Cmaq_1224 antibody

Western blot analysis of RFK (arrow) using rabbit polyclonal RFK Antibody (C15) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFK gene.
Rabbit Anti-Cmaq_1224 Polyclonal Antibody, Western Blot (WB) – Cat# MBS630969

Flow Cytometry (FC/FACS) of anti-Cmaq_1224 antibody

RFK Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-Cmaq_1224 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS630969

Other Notes

Small volumes of anti-Cmaq_1224 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cmaq_1224 purchase

MBS630969 is an antibody targeting Cmaq_1224. Also known as RFK, NT (Riboflavin Kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Cmaq_1224. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Riboflavin kinase
NCBI Official Symbol
Cmaq_1224
UniProt Protein Name
Riboflavin kinase
UniProt Synonym Protein Names
CTP-dependent riboflavin kinase; CTP:riboflavin 5'-phosphotransferase; Flavokinase
UniProt Gene Name
ribK
UniProt Synonym Gene Names
RFK
UniProt Entry Name
RIFK_CALMQ
NCBI Protein Information
hypothetical protein

UniProt Comments for Cmaq_1224

Function: Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN)

By similarity. HAMAP-Rule MF_01285

Catalytic activity: CTP + riboflavin = CDP + FMN. HAMAP-Rule MF_01285

Cofactor: Binds 1 magnesium ion per subunit

By similarity. HAMAP-Rule MF_01285

Pathway: Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP-Rule MF_01285

Sequence similarities: Belongs to the archaeal riboflavin kinase family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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