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INSC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6310601

In Drosophila, neuroblasts divide asymmetrically into another neuroblast at the apical side and a smaller ganglion mother cell on the basal side. Cell polarization is precisely regulated by 2 apically localized multiprotein signaling complexes that are… More details

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Product Overview

Online Datasheet in PDF
Product Name
INSC, Polyclonal Antibody
Full Product Name
INSC, CT (INSC, Protein inscuteable homolog) (Azide free) (HRP)
Product Gene Name
Product Synonym Names
INSC; Protein inscuteable homolog
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
63,469 Da
OMIM
610668
3D Structure
ModBase 3D Structure for Q1MX18
Immunogen
INSC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 416-445 amino acids from the C-terminal region of human INSC.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-INSC antibody

Applications Tested/Suitable for anti-INSC antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-INSC antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-INSC antibody

INSC Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the INSC antibody detected the INSC protein (arrow).
Rabbit Anti-INSC Polyclonal Antibody – Cat# MBS6310601

Immunohistochemistry (IHC) of anti-INSC antibody

INSC antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INSC antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-INSC Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6310601

Product References and Citations for anti-INSC antibody

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Vural, A., et al. Mol. Cell. Biol. 30(6):1528-1540(2010). Rivadeneira, F., et al. Nat. Genet. 41(11):1199-1206(2009). Izaki, T., et al. Biochem. Biophys. Res. Commun. 341(4):1001-1006(2006). Katoh, M., et al. Int. J. Mol. Med. 11(1):111-116(2003).

Other Notes

Small volumes of anti-INSC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for INSC purchase

MBS6310601 is an antibody targeting INSC. Also known as INSC, CT (INSC, Protein inscuteable homolog) (Azide free) (HRP). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for INSC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein inscuteable homolog isoform a
NCBI Official Synonym Full Names
inscuteable homolog (Drosophila)
NCBI Official Symbol
INSC
UniProt Protein Name
Protein inscuteable homolog
Protein Family
UniProt Gene Name
INSC
UniProt Entry Name
INSC_HUMAN
NCBI Protein Information
protein inscuteable homolog; inscuteable spindle orientation adaptor protein

UniProt Comments for INSC

INSC: May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex. Involved in spindle orientation during mitosis it may regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: apical part of cell; cytoplasm

Biological Process: nervous system development; establishment of mitotic spindle orientation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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