• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CYP51A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631097

CYP51A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum… More details

$895 / 0.2 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CYP51A1, Polyclonal Antibody
Full Product Name
CYP51A1, ID (Cytochrome P450-14DM, Cytochrome P45014DM, Sterol 14-alpha Demethylase, Cytochrome P450LI, CYPLI, Cytochrome P450 51A1, Lanosterol 14-alpha Demethylase, LDM, CYP51)
Product Gene Name
Product Synonym Names
Anti -CYP51A1, ID (Cytochrome P450-14DM, Cytochrome P45014DM, Sterol 14-alpha Demethylase, Cytochrome P450LI, CYPLI, Cytochrome P450 51A1, Lanosterol 14-alpha Demethylase, LDM, CYP51)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human CYP51A1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
19,537 Da
Chromosome Location
Chromosome: 7; NC_000007.13 (91741463..91764059, complement). Location: 7q21.2
3D Structure
ModBase 3D Structure for Q05D40
Immunogen
Synthetic peptide selected from the Center region of human CYP51A1 (KLH).
Applications Tested/Suitable for anti-CYP51A1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-CYP51A1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-CYP51A1 antibody

Western blot analysis of CYP51A1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). CYP51A1 (arrow) was detected using the purified Pab.
Rabbit Anti-CYP51A1 Polyclonal Antibody – Cat# MBS631097

Immunohistochemistry (IHC) of anti-CYP51A1 antibody

Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-CYP51A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS631097

Flow Cytometry (FC/FACS) of anti-CYP51A1 antibody

CYP51A1 Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CYP51A1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS631097

Other Notes

Small volumes of anti-CYP51A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP51A1 purchase

MBS631097 is an antibody targeting CYP51A1. Also known as CYP51A1, ID (Cytochrome P450-14DM, Cytochrome P45014DM, Sterol 14-alpha Demethylase, Cytochrome P450LI, CYPLI, Cytochrome P450 51A1, Lanosterol 14-alpha Demethylase, LDM, CYP51). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CYP51A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
CYP51A1 protein
NCBI Official Synonym Full Names
cytochrome P450, family 51, subfamily A, polypeptide 1
NCBI Official Symbol
CYP51A1
NCBI Official Synonym Symbols
LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM
UniProt Protein Name
CYP51A1 protein
UniProt Gene Name
CYP51A1
UniProt Entry Name
Q05D40_HUMAN
NCBI Protein Information
lanosterol 14-alpha demethylase; CYPLI; cytochrome P450LI; OTTHUMP00000161775; OTTHUMP00000208817; cytochrome P45014DM; cytochrome P450 51A1; cytochrome P450-14DM; lanosterol 14-alpha-demethylase; cytochrome P450, 51 (lanosterol 14-alpha-demethylase)

NCBI Summary for CYP51A1

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Show more

UniProt Comments for CYP51A1

CYP51A1: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - steroid biosynthesis; Oxidoreductase; Membrane protein, integral; EC 1.14.13.70

Chromosomal Location of Human Ortholog: 7q21.2

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: sterol 14-demethylase activity; iron ion binding; heme binding

Biological Process: xenobiotic metabolic process; sterol metabolic process; cholesterol biosynthetic process; steroid biosynthetic process; cholesterol biosynthetic process via 24,25-dihydrolanosterol
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000